Barrick Lab :: Laboratory Protocols
Creating Protocols
Lab Stocks and Practices
PCR and Sequencing
Genetic Modification and Cloning
Mutation Rates, Growth Rates and Evolutionary Stability
- Fluctuation Tests
Measure bacterial mutation rates to antibiotic resistance, phage resistance, or reversion of auxotrophy.
- Growth Rates
Measure bacterial growth from OD600/platereader data and calculate growth rates with the Growthcurver R package.
- Plasmid Copy Number
Measure copy number of plasmids compared to genome.
- Fluorescent Protein Evolutionary Stability
Measure the evolutionary stability of fluorescent protein expression.
- CFU Counts
Measure the number of CFU in a sample with this simple spot-plating method
Phage
Genome Resequencing and Mutation Validation
Experimental Evolution
Flow Cytometry and Cell Sorting
Nucleic Acid Preparation, RNA-Seq, qPCR
Protein Expression and Purification
in vitro Enzyme Activity Assays
Working with Unusual Microbes
Working with Insects
Acinetobacter baylyi ADP1
Useful Resources
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Topic revision: r264 - 2024-04-09 - LucioNavarro