Protocol Updates Needed

  • this page on DNA sequencing needs to be updated and be made more for the general public than to the lab
  • General guide for sorting bacteria. This information is not widely available and I had to consult r/flowcytometry to find out. (Tyler)
    • Things like performing a dilution vs eps calibration curve to avoid swarming, sample prep, etc.
  • Updating fluctuation test page to include better tools for calculating mutation rate (mlemur). (Tyler)
  • Add a page for purifying and storing HMW DNA (Isaac)
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep).
    • Ideally, also include a calculation of how many cycles are needed.
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Update research interests page (Jeff)
  • Anaconda should not be recommended
  • Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
  • Add information about Lactobacillus strains in bees.
Edit | Attach | Watch | Print version | History: r41 < r40 < r39 < r38 < r37 | Backlinks | Raw View | More topic actions

 Barrick Lab  >  ProtocolList  >  ProtocolUpdatesNeeded

Contributors to this topic Edit topic JeffreyBarrick, TylerDeJong, DanielDeatherage, CraigBarnhart, SeanLeonard, CameronRoots, AurkoDasgupta, JordanMonk, LindseyWolf, AlvaroRodriguez, SimonDAlton, ZuberiAshraf, SimonDOelsnitz, IsaacGifford
Topic revision: r41 - 2025-03-20 - 21:04:16 - Main.CameronRoots
 
This site is powered by the TWiki collaboration platform Powered by Perl This site is powered by the TWiki collaboration platformCopyright ©2025 Barrick Lab contributing authors. Ideas, requests, problems? Send feedback