Protocol Updates Needed
- this page on DNA sequencing needs to be updated and be made more for the general public than to the lab
- General guide for sorting bacteria. This information is not widely available and I had to consult r/flowcytometry to find out. (Tyler)
- Things like performing a dilution vs eps calibration curve to avoid swarming, sample prep, etc.
- Updating fluctuation test page to include better tools for calculating mutation rate (mlemur). (Tyler)
- Add a page for purifying and storing HMW DNA (Isaac)
- Update PCR protocol to emphasize not adding too much template (ex: miniprep).
- Ideally, also include a calculation of how many cycles are needed.
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Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
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Add a page with standard plate reader settings for different fluorophores (with citations)
- Also explain setting the gain!
- Add a page describing tools for plotting and fitting growth curves
- Standard PCR – add troubleshooting information here?
- Page with books/resources for students joining the lab? (Lucio)
- Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
- Nuke most topics under computation and many under reference (Dan)
- Link all the golden gate materials (and work on general GGA materials)
- PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
- Migrate LTEE stuff to LTEE website (Jeff)
- Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
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Computation tool list -- Add pLannotate and OSTIR (Cameron)
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Team – add start dates, be sure no former lab members were not transferred there.
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Update research interests page (Jeff)
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Anaconda should not be recommended
- Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
- Add information about Lactobacillus strains in bees.
Barrick Lab > ProtocolList > ProtocolUpdatesNeeded
Contributors to this topic

JeffreyBarrick, TylerDeJong, DanielDeatherage, CraigBarnhart, SeanLeonard, CameronRoots, AurkoDasgupta, JordanMonk, LindseyWolf, AlvaroRodriguez, SimonDAlton, ZuberiAshraf, SimonDOelsnitz, IsaacGifford
Topic revision: r41 - 2025-03-20 - 21:04:16 - Main.CameronRoots