Protocol Updates Needed

• this page on DNA sequencing needs to be updated and be made more for the general public than to the lab
• General guide for sorting bacteria. This information is not widely available and I had to consult r/flowcytometry to find out. (Tyler)
  • Things like performing a dilution vs eps calibration curve to avoid swarming, sample prep, etc.
• Updating fluctuation test page to include better tools for calculating mutation rate (mlemur). (Tyler)
• Add a page for purifying and storing HMW DNA (Isaac)
• Update PCR protocol to emphasize not adding too much template (ex: miniprep).
  • Ideally, also include a calculation of how many cycles are needed.
• Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
• Add a page with standard plate reader settings for different fluorophores (with citations)
  • Also explain setting the gain!
• Add a page describing tools for plotting and fitting growth curves
• Standard PCR – add troubleshooting information here?
• Page with books/resources for students joining the lab? (Lucio)
• Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
• Nuke most topics under computation and many under reference (Dan)
• Link all the golden gate materials (and work on general GGA materials)
• PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
• Migrate LTEE stuff to LTEE website (Jeff)
• Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
• Computation tool list -- Add pLannotate and OSTIR (Cameron)
• Team – add start dates, be sure no former lab members were not transferred there.
• Update research interests page (Jeff)
• Anaconda should not be recommended
• Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
• Add information about Lactobacillus strains in bees.
• Troubleshooting minipreps, handling isolation of large plasmids