50 Recent Changes in Lab Web retrieved at 10:03 (GMT)

Main.TWikiGuest

Statistics for nop Lab Web Month: Topic views: Topic saves: File uploads: Most popular topic views: Top contributors for topic save and ...
Main.PengGeng

in vitro RNA synthesis(T7 RNA Polymerase ) T7 RNA Polymerase is used for in vitro mRNA synthesis and is highly specific for the T7 phage promoter. T7 in vitro transcription ...
Main.PengGeng

Barrick Lab :: Laboratory Protocols Creating Protocols How to Create a Protocol Best practices for designing a protocol and for putting it on the lab Wiki. ...
DNA Concentration Determination For many applications in cloning and genome editing, it is critical to accurately measure the concentration of DNA in a sample. This ...
Main.ElizabethRobinson

Tobacco hornworm (Manduca sexta) care The tobacco hornworm is the caterpillar stage of the five spotted hawk moth ( Manduca sexta ). They constitute a major pest of ...
Competence Assays A competence assay quantifies the ability of bacterial strains to uptake DNA in culture. The protocol below utilizes genomic DNA extracts obtained ...
Read trimming with trimmomatic Download and Install Download the "binary" version of trimmomatic from the lab. It's helpful to a create a shortcut so that you ...
Analyzing RNA Seq data for differential gene expression Materials and Software Data files RNA Seq data in FASTQ format (Ex: dataset1.fastq, dataset2 ...
Main.JeffreyBarrick

Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate ...
Keio and ASKA collections Dear internet, Our lab is unable to distribute the Keio/ASKA strains to other institutions. We did not create them! If you want to use ...
Main.KateElston

Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick #128011 ...
Acinetobacter baylyi ADP1 Golden Transformation Overview The following protocol is to be used as a substitute for overlap extension PCR for constructing double stranded ...
Isolating Phage Genomic DNA This protocol has been tested with phage T7. It has a double stranded genome that has a length of 40 kb. Materials 5× phage precipitation ...
Main.JeffreyBarrick

The University of Texas at Austin :: iGEM Team Interested in joining the UT Austin iGEM Team? Participating in the iGEM competition is a unique opportunity for undergraduate ...
General Conjugation Protocol Return to BTK Page Conjugation is a reliable, robust method to transfer plasmids between bacteria. This is a general purpose protocol ...
Main.JeffreyBarrick

Determining Phage Titer Materials Phage lysate or freezer stock (Procedure: Phage Lysate Preparation) Top Agar (LB Miller 0.8% agar) LB MIller (for ...
Main.JeffreyBarrick

Phage Lysate Preparation We have used this protocol with phages T4, T5, T6 and T7. The general procedure should work with most E. coli phages (although lysis times ...
Main.JeffreyBarrick

Past Barrick Lab Members Gabriel Suarez Grace Stark Stratton Georgoulis Jenna McGuffey Dr. Simon D'Alton Dacia Leon Colin Brown Xue Zhang Michael Hammerling Brian ...
Main.JeffreyBarrick

Barrick Lab :: Contact Information Jeffrey E. Barrick, Ph.D. Associate Professor Department of Molecular Biosciences The University of Texas at Austin Office ...
Main.KateElston

C1 Condition 1, C2 Condition 2, and BR# Biological Replicate Template Material Use cDNA dilution determined in cDNA Concentration qPCR What you are looking ...
Main.ElizabethRobinson

Autoclave Sterilization Overview Autoclaves heat their contents to 121°C, which is 21°C over the boiling point of water. To prevent the solutions from boiling over ...
(Numbers are PCR product dilution with "10" representing a 1:10 dilution of your 0.01ng/ul PCR product prepped as described above) Template Material Use PCR ...
(Numbers are cDNA dilution with "5" representing a 1:5 dilution and so on) Template Material The cDNA used for this is a pool of all your cDNA samples i.e. ...
Main.JeffreyBarrick

Barrick Lab ' '' " then "" else ""}% Contact Research Publications Team Protocols Reference Computation ...
Media amendments Antibiotic concentrations and stock solutions This table lists “standard” concentrations of antibiotics commonly used in microbiology laboratories ...
Unix Commands Quick Reference Useful commands and flags that we get tired of looking up... Disk Space The du command is verbose and confusing if you run it without ...
Main.JeffreyBarrick

Media Recipes Liquid Media Included topic: ProtocolsRecipesSaline Included topic: ProtocolsRecipesLysogenyBroth Included topic: ProtocolsRecipesDavisMingioli ...
Transforming Acinetobacter baylyi ADP1 About A. baylyi ADP1 Acinetobacter baylyi ADP1 is a naturally competent bacteria with enormous potential for genome engineering ...
Major version notes Runs prior to February 2020 had a variety of formats and sample submission requirements. Suggested that individual runs be consulted on utbox for ...
Main.MattMcGuffie

...in progress...
Main.MattMcGuffie

Barrick Lab :: Code Also check out our code repository: Github/BarrickLab Breseq (Bacterial Genome Resequencing)Determine mutations in evolved bacteria from next ...
LB: Lysogeny Broth / Luria Bertani (Miller) Agar Combine in a 2 L flask the following: 1.5L Component 15 g Tryptone 7.5 g Yeast Extract ...
Main.MattMcGuffie

Copying files to/from UT Box at the command line Follow the directions here to set up an additional Box password that you will use for connecting: https://uisapp2 ...
Main.SarahBialik

Sensaphone Protocol The sensaphone is the alarm monitor connected to both of the 80C freezers in MBB. Below is an overview of the current settings and the expected ...
Main.JeffreyBarrick

Barrick Lab :: Computational Protocols Command line Unix Commands Quick Reference Collection of useful Unix commands. SSH Public Key Authentication How ...
Main.JeffreyBarrick

Working with Git Setup GitHub Ask to be granted access to see private repositories Learning Git it – App for teaching you to use command line Git on ...
Main.JeffreyBarrick

Interested in research in the Barrick lab? We are always looking for outstanding undergraduate students, graduate students, and postdocs who are interested in experimental ...
Working with Vibrio natriegens (Vmax) Vibrio natriegens has the fastest doubling time of any known organism and has the potential to shorten experimental timelines ...
Main.JeffreyBarrick

ABMS: Acinetobacter Minimal Succinate Courtesy of the Averhoff lab, with modifications for available reagents. To make standard ABMS, combine the following pre sterilized ...
Back to Golden Gate Protocols Troubleshooting Golden Gate Assemblies Tips Screen Inserts for internal BsaI/BsmBI sites! Reactions with single off target sites ...
Protocol for harvesting Pfu Sso7d polymerase This protocol is for expressing and purifying the Pfu Sso7d polymerase from E. coli 1 . A variant of this protein with ...
P1 Transduction in Eschericha coli This protocol is adapted from Thomason, L. C., Costantino, N. and Court, D. L. 2007. E. coli Genome Manipulation by P1 Transduction ...
Gene Gorging Marker Mutations For competitive fitness assays, one must be able to distinguish two E. coli subpopulations in a mixed culture. One way this can be ...
Freezing Strains Supplies 1 Overnight liquid culture Several milliliters of overnight liquid culture grown in a suitable medium for each sample to be frozen ...
Main.KateElston

Quantitative real time PCR for differential gene expression THIS PAGE IS CURRENTLY UNDER CONSTRUCTION APOLOGIES FOR ANYTHING THAT'S STILL A BIT MESSY! Quantitative ...
RCV Medium RCV is a complex media consisting of 3 different sub components that must be prepared ahead of time if they are not already made. Main recipe 1L ...
Main.KateElston

1 2 3 4 5 6 7 8 9 A C1BR1 C1BR1 C1BR1 C1BR1 C1BR1 C1BR1 C1BR1 C1BR1 C1BR1 B C1BR2 C1BR2 ...
TA: Tetrazolium Sugar (TA, TM, TL, ...) Combine in a 2 L flask: 1.5L Component 15 g Tryptone 1.5 g Yeast Extract 7.5 g NaCl 24 ...
Major version notes This is a protocol based on Kapa LTP Preparation Kit manual KR0453 v3.13 The main difference centers around the use of 1/2 volume in each reaction ...
Fluctuation Tests Introduction This protocol is for doing a Luria Delbrück fluctuation test to measure the rate at which mutations occur that enable growth on selective ...
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Contributors to this topic edittopic TWikiContributor, PeterThoeny
Topic revision: r2 - 28 Mar 2005 - 09:40:13 - Main.TWikiContributor
 
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