Difference: GeneratingOverhangs (1 vs. 4)
Revision 42025-06-17 - AlexaMorton
Generating Overhangs | ||||||||
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| < < | If generating overhangs for Golden Gate Assembly outside of YTK/BTK framework, NEB's GETSET tool can be useful for generating a set of high fidelity overhangs to use. If compatibility with other overhangs is necessary required overhangs may be set. | |||||||
| > > | If generating overhangs for Golden Gate Assembly outside of YTK/BTK framework, NEB's GETSET tool can be useful for generating a set of high fidelity overhangs to use. If compatibility with other overhangs is necessary required overhangs may be set. | |||||||
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| < < | For scarless assembly NEB's SPLITSET tool may be used. You'll need to specify the DNA sequence you're assembling, and how it will be broken up into fragments. The tool will find high fidelity overhangs that will allow you to scarlessly assembly your DNA using Golden Gate Assembly. | |||||||
| > > | For scarless assembly NEB's SPLITSET tool may be used. You'll need to input the DNA sequence you're assembling and specify the number of assembly fragments. The tool will find high fidelity overhangs that will allow you to scarlessly assembly your DNA using Golden Gate Assembly. | |||||||
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| > > | These tools can be useful for domesticating part plasmids, for creating high-fidelity subparts for an existing assembly schema (such as the BTK/YTK), and for creating scarless assemblies. | |||||||
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| < < | --  Victor Li - 2021-11-03 | |||||||
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| < < | Comments | |||||||
| > > | -- Victor Li - 2021-11-03 -- Updated by Alexa Morton - 2025-06-17 | |||||||
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Revision 32021-11-04 - VictorLi
Generating OverhangsIf generating overhangs for Golden Gate Assembly outside of YTK/BTK framework, NEB's GETSET tool can be useful for generating a set of high fidelity overhangs to use. If compatibility with other overhangs is necessary required overhangs may be set. For scarless assembly NEB's SPLITSET tool may be used. You'll need to specify the DNA sequence you're assembling, and how it will be broken up into fragments. The tool will find high fidelity overhangs that will allow you to scarlessly assembly your DNA using Golden Gate Assembly. -- Victor Li - 2021-11-03
Comments |
Revision 22021-11-03 - VictorLi
Generating Overhangs | ||||||||
| Added: | ||||||||
| > > | If generating overhangs for Golden Gate Assembly outside of YTK/BTK framework, NEB's GETSET tool can be useful for generating a set of high fidelity overhangs to use. If compatibility with other overhangs is necessary required overhangs may be set. For scarless assembly NEB's SPLITSET tool may be used. You'll need to specify the DNA sequence you're assembling, and how it will be broken up into fragments. The tool will find high fidelity overhangs that will allow you to scarlessly assembly your DNA using Golden Gate Assembly. | |||||||
-- Victor Li - 2021-11-03
Comments | ||||||||
Revision 12021-11-03 - VictorLi
Generating Overhangs-- Victor Li - 2021-11-03
Comments |
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