(1 vs. 8) ProtocolsRecipesTetrazoliumSugar < Lab

Revision 82025-01-09 - AlexaMorton

META TOPICPARENT	name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Changed:

<
<

Combine in a 2 L flask:

1.5L	Component
15 g	Tryptone
1.5 g	Yeast Extract
7.5 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.3 liter.

>
>

Combine in a 1 L flask:

500 mL	Component
5 g	Tryptone
500 mg	Yeast Extract
2.5 g	NaCl
8 g	Agar
500 µL	5% Antifoam

Add dH₂O to 433.33 mL.

Changed:

<
<

Combine in a 500 ml flask:

1.5L	Component
15 g	Sugar (arabinose = TA, maltose = TM, lactose = TL)

Add dH₂O to 0.2 liter.

>
>

Combine in a 125 ml flask:

500 mL	Component
5 g	Sugar (arabinose = TA, maltose = TM, lactose = TL)

Add dH₂O to 66.67 mL.

Changed:

<
<

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

>
>

Cover each flask with foil, and autoclave sugar and media separately. Total combined volume will be 500 mL.

Changed:

<
<

Combine and add:

1.5L	Component
1.5 ml	5% (w/v) Triphenyl Tetrazolium chloride (TTC), filter sterilized, stored at 4°C

>
>

Combine the autoclaved sugar and media solutions and add:

500 mL	Component
500 µL	5% (w/v) Triphenyl Tetrazolium chloride (TTC), filter sterilized, stored at 4°C

Source: Lenski Lab Protocol

Revision 72019-03-08 - JeffreyBarrick

META TOPICPARENT	name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

1.5L	Component
15 g	Tryptone
1.5 g	Yeast Extract
7.5 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.3 liter.

Combine in a 500 ml flask:

1.5L	Component
15 g	Sugar (arabinose = TA, maltose = TM, lactose = TL)

Add dH₂O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5L	Component

Changed:

<
<

1.5 ml

5% (w/v) Tetrazolium chloride (TTC), filter sterilized, stored at 4°C

>
>

1.5 ml

5% (w/v) Triphenyl Tetrazolium chloride (TTC), filter sterilized, stored at 4°C

Source: Lenski Lab Protocol

Revision 62018-10-25 - JeffreyBarrick

META TOPICPARENT	name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

1.5L	Component
15 g	Tryptone
1.5 g	Yeast Extract
7.5 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.3 liter.

Combine in a 500 ml flask:

1.5L	Component
15 g	Sugar (arabinose = TA, maltose = TM, lactose = TL)

Add dH₂O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5L	Component

Changed:

<
<

1.5 ml

TTC (5%)

>
>

1.5 ml

5% (w/v) Tetrazolium chloride (TTC), filter sterilized, stored at 4°C

Source: Lenski Lab Protocol

Revision 52015-03-21 - JordanMonk

META TOPICPARENT	name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

1.5L	Component
15 g	Tryptone
1.5 g	Yeast Extract
7.5 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.3 liter.

Combine in a 500 ml flask:

1.5L	Component

Changed:

<
<

6 g	Sugar (arabinose = TA, maltose = TM, lactose = TL)

>
>

15 g	Sugar (arabinose = TA, maltose = TM, lactose = TL)

Add dH₂O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5L	Component
1.5 ml	TTC (5%)

Source: Lenski Lab Protocol

Revision 42012-05-04 - JeffreyBarrick

META TOPICPARENT	name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

1.5L	Component
15 g	Tryptone
1.5 g	Yeast Extract
7.5 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.3 liter.

Combine in a 500 ml flask:

1.5L	Component
6 g	Sugar (arabinose = TA, maltose = TM, lactose = TL)

Add dH₂O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5L	Component

Changed:

<
<

1.5 ml

TTC (5%)

or

7.5 ml

TTC (1%)

>
>

1.5 ml

TTC (5%)

Added:

>
>

Source: Lenski Lab Protocol

Revision 32011-07-29 - CraigBarnhart

META TOPICPARENT	name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Added:

>
>

Combine in a 2 L flask:

Added:

>
>

1.5L	Component

15 g	Tryptone
1.5 g	Yeast Extract
7.5 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.3 liter.

Combine in a 500 ml flask:

Added:

>
>

1.5L	Component

6 g	Sugar (arabinose = TA, maltose = TM, lactose = TL)

Add dH₂O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

Added:

>
>

1.5L	Component

1.5 ml

TTC (5%)

or

7.5 ml

TTC (1%)

Revision 22011-07-26 - CraigBarnhart

META TOPICPARENT	name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

15 g	Tryptone
1.5 g	Yeast Extract
7.5 g	NaCl
24 g	Agar

Changed:

<
<

1.5 ml

5% Antifoam

or

.75 ml

10% Antifoam

>
>

1.5 ml

5% Antifoam

Add dH₂O to 1.3 liter.

Combine in a 500 ml flask:

6 g	Sugar (arabinose = TA, maltose = TM, lactose = TL)

Add dH₂O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5 ml

TTC (5%)

or

7.5 ml

TTC (1%)

Revision 12011-06-14 - CraigBarnhart

META TOPICPARENT	name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

15 g	Tryptone
1.5 g	Yeast Extract
7.5 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam	or	.75 ml	10% Antifoam

Add dH₂O to 1.3 liter.

Combine in a 500 ml flask:

6 g	Sugar (arabinose = TA, maltose = TM, lactose = TL)

Add dH₂O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5 ml

TTC (5%)

or

7.5 ml

TTC (1%)

Difference: ProtocolsRecipesTetrazoliumSugar (1 vs. 8)

Revision 82025-01-09 - AlexaMorton

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Revision 72019-03-08 - JeffreyBarrick

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Revision 62018-10-25 - JeffreyBarrick

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Revision 52015-03-21 - JordanMonk

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Revision 42012-05-04 - JeffreyBarrick

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Revision 32011-07-29 - CraigBarnhart

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Revision 22011-07-26 - CraigBarnhart

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Revision 12011-06-14 - CraigBarnhart

TA: Tetrazolium Sugar (TA, TM, TL, ...)