Generating Overhangs

If generating overhangs for Golden Gate Assembly outside of YTK/BTK framework, NEB's GETSET tool can be useful for generating a set of high fidelity overhangs to use. If compatibility with other overhangs is necessary required overhangs may be set.

For scarless assembly NEB's SPLITSET tool may be used. You'll need to specify the DNA sequence you're assembling, and how it will be broken up into fragments. The tool will find high fidelity overhangs that will allow you to scarlessly assembly your DNA using Golden Gate Assembly.

-- Victor Li - 2021-11-03