LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

Combine in a 2 L flask the following:

1.5L Component
15 g Tryptone
7.5 g Yeast Extract
15 g NaCl
24 g Agar
1.5 ml 5% Antifoam

Add dH2O to 1.5 L. Autoclave.

Notes and variations:

  • Common synonyms are Luria-Broth and Luria-Bertani medium.
  • This is the "Miller" formulation of LB with 10 g NaCl per liter (1% w/v). Another common formulation has only 5 g NaCl per liter (0.5% w/v)! They are NOT interchangeable.
  • If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
  • To make "soft" or "top" agar for phage studies, use only 7-8 g agar per liter.
Edit | Attach | Watch | Print version | History: r8 < r7 < r6 < r5 < r4 | Backlinks | Raw View | More topic actions

 Barrick Lab  >  ProtocolsMediaRecipes  >  ProtocolsRecipesLysogenyBrothAgar

Contributors to this topic Edit topic JeffreyBarrick, CraigBarnhart, AlvaroRodriguez
Topic revision: r8 - 2020-02-11 - 20:28:28 - Main.JeffreyBarrick
Lab.ProtocolsRecipesLysogenyBrothAgar moved from Lab.ProtocolsRecipesLysogenyBroth on 2015-06-23 - 14:17 by Main.JeffreyBarrick -
This site is powered by the TWiki collaboration platform Powered by Perl This site is powered by the TWiki collaboration platformCopyright ©2023 Barrick Lab contributing authors. Ideas, requests, problems? Send feedback