The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise. https://barricklab.org/twiki/bin/view/Lab Copyright 2026 by contributing authors 2026-05-13T15:26:23Z https://barricklab.org/twiki/bin/view/Lab/ReferencePlasmids 2026-05-13T15:26:23Z

Commonly Used Plasmids plasmid markers origin host ref description pCA24N CamR Kitagawa2005 ASKA collection vector pKD... (last changed by IraZibbu)

IraZibbu https://barricklab.org/twiki/bin/view/Lab/ListofBioinformatics 2026-05-13T15:17:01Z

Bioinformatics Master List This page is a master list of bioinformatics software frequently used in the lab, and their purposes. It also includes additional notes... (last changed by IraZibbu)

IraZibbu https://barricklab.org/twiki/bin/view/Lab/ProtocolList 2026-05-13T15:12:01Z

Barrick Lab :: Laboratory Protocols Updating Protocols How to Create a Protocol Best practices for designing a protocol and for putting it on the lab Wiki.... (last changed by PraneshRao)

PraneshRao https://barricklab.org/twiki/bin/view/Lab/ProtocolsBTKAssembleSingleTUPlasmid 2026-05-13T15:11:40Z

Back to Golden Gate Protocols First Stage Plasmid (Transcriptional Unit) Assembly Once you have all your desired part plasmids built you can assemble them into Transcriptional... (last changed by AnthonyVandieren)

AnthonyVandieren https://barricklab.org/twiki/bin/view/Lab/WebHome 2026-05-13T15:07:29Z

.within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by PraneshRao)

PraneshRao https://barricklab.org/twiki/bin/view/Lab/Strain_side_notes 2026-05-13T15:01:32Z

Commonly Used E. coli Strains OpenWetWare offers a comprehensive repository of the genotypes of the most commonly used E. coli strains. The following table points... (last changed by IraZibbu)

IraZibbu https://barricklab.org/twiki/bin/view/Lab/WebStatistics 2026-05-13T13:22:57Z

Statistics for Lab Web Month: Topic views: Topic saves: File uploads: Most popular topic views: Top contributors for topic save and... (last changed by TWikiGuest)

TWikiGuest https://barricklab.org/twiki/bin/view/Lab/PublicationList 2026-05-09T10:31:05Z

Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Legend: Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick)

JeffreyBarrick https://barricklab.org/twiki/bin/view/Lab/LabPersonnel 2026-05-01T12:29:39Z

Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick)

JeffreyBarrick https://barricklab.org/twiki/bin/view/Lab/UTiGEMTeam 2026-04-30T10:09:33Z

The University of Texas at Austin :: iGEM Team The Barrick lab sponsored and mentored the UT Austin iGEM team from 2012 2025 when we were located at The University... (last changed by JeffreyBarrick)

JeffreyBarrick https://barricklab.org/twiki/bin/view/Lab/ProtocolsElectrocompetentCells 2026-04-29T13:52:59Z

Electrocompetent E. coli Making Electrocompetent E. coli Cells (small batch) This procedure makes enough electrocompetent cells for 2 3 transformations. 1 Grow... (last changed by IraZibbu)

IraZibbu https://barricklab.org/twiki/bin/view/Lab/Protocols16SSequencing 2026-04-28T19:00:14Z

16S rRNA Sequencing to Identify Unknown Microbes Ever wonder what that contaminant in your culture is? Need to accurately identify an environmental isolate? Overview... (last changed by JeffreyBarrick)

JeffreyBarrick https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesABMS 2026-04-28T00:45:40Z

ABMS: Acinetobacter Minimal Succinate Courtesy of the Averhoff lab, with modifications for available reagents. To make standard ABMS, combine the following pre sterilized... (last changed by JeffreyBarrick)

JeffreyBarrick https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesDavisMingioli 2026-04-22T18:39:45Z

DM: Davis Mingioli Growth medium used by the long term E. coli evolution experiment. 0.5L 1L 4.5L 5L Component MW 2.67 g... (last changed by JeffreyBarrick)

JeffreyBarrick https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesSOB 2026-04-22T17:15:10Z

SOB/SOC: Super Optimal Broth For SOB: 200mL 250mL 1L Final Component 1 g 1.25 g 5 g 0.5% yeast extract... (last changed by JeffreyBarrick)

JeffreyBarrick https://barricklab.org/twiki/bin/view/Lab/PastLabPersonnel 2026-04-17T02:33:11Z

Past Barrick Lab Members Devin Lake Lucio Navarro Vibhav Iyengar Anna Grove Krisha Chaudhari Korin Jones Dr. Patrick Lariviere Dr. Dennis Mishler Alexa Morton Cameron... (last changed by JeffreyBarrick)

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