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Creating Protocols

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Lab Stocks and Practices

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PCR and Sequencing

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Genetic Modification and Cloning

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Bioinformatics

Environmental Microbiology

Writing and Presenting

 

Useful Resources

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Creating Protocols

 
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Genetic Modification

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Genetic Modification and Cloning

 
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  • Restriction Enzyme Cloning
    Insert a target DNA sequence into a plasmid via restriction digest and ligation.
  • Gene Gorging Neutral Markers
    λ red genetic modification for mutations with a selectable or screenable marker using donor plasmid. For example, converting Ara+ strains to Ara–.
  • Gene Gorging Evolved Alleles
    λ red genetic modification without a selectable or screenable phenotype using donor plasmid.
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  • FLP-FRT Recombination
    Induce targeted recombination (commonly used to eliminate the KanR cassette Keio strains).
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  • FLP-FRT Recombination
    Induce targeted recombination (commonly used to eliminate the KanR cassette from Keio strains).
 
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Experimental Evolution

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Barrick Lab :: Protocols and Laboratory Reference

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Lab Stocks

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Protocols and Laboratory Reference

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Stock Solutions

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Lab Stocks

 
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PCR

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PCR and Sequencing

 
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Sequence Analysis

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Microbiology

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Genetic Modification

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Mutation Rates

 
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  • Fluctuation Tests
    Measure bacterial mutation rates to antibiotic resistance, phage resistance, or reversion of auxotrophy.
  • Phage Stocks
    Phage Lysate Preparation (T4,T5,T6) and Determining Phage Titer
 

Genome Re-sequencing and Mutation Validation

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Experimental Evolution of Bacteria

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Experimental Evolution

 
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See Also

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