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| META TOPICPARENT |
name="ProtocolsMediaRecipes" |
MOB: Mobility Media
This media is useful for enhancing the mobility of ADP1, which moves across the plate by "twitching".
| 1L |
Final [ ] |
Component |
| 5 g |
0.5% |
agarose |
| 5 g |
2.0% |
tryptone |
| 2.5 g |
10 mM |
NaCl |
|
|
<
< | After pouring your gel, let it set overnight at room temperature. The next day, spot with 1uL of your overnight-grown culture, leave the plate to dry for 15 minutes at room temperature, wrap in Parafilm, invert, and incubate at 30°C. You should see spreading over the next 24 hours. |
>
> | After pouring your gel, let it set overnight at room temperature. The next day, spot with 1µl of your overnight-grown culture, leave the plate to dry for 15 minutes at room temperature, wrap in Parafilm, invert, and incubate at 30°C. You should see spreading over the next 24 hours. |
