---+ Commonly Used Plasmids | *plasmid* | *markers* | *origin* | *host* | *ref* | *description* | | pCA24N | Cam^R | | | [[http://dnaresearch.oxfordjournals.org/content/12/5/291.long][Kitagawa2005]] | ASKA collection vector | | pKD46 | Amp^R | TS | ? | [[http://www.ncbi.nlm.nih.gov/pubmed/10829079][Datsenko&Wanner2000]] | Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number. | | pKD78 | Cam^R | TS | | | Identical plasmid to pKD46, but with CAM resistance instead of AMP.| | pKD3 | Cam^R | | BW25141 | [[http://www.ncbi.nlm.nih.gov/pubmed/10829079][Datsenko&Wanner2000]] | Wanner template plasmid | | pKD13 | Kan^R | | BW25141 | [[http://www.ncbi.nlm.nih.gov/pubmed/10829079][Datsenko&Wanner2000]] | Wanner template plasmid | | pCR2.1 | Kan^R, Amp^R | | | | TOPO cloning kit vector | | pACYC184 | Tet^R, Cam^R | | ER2420 | [[http://jb.asm.org/content/134/3/1141.full.pdf+html][Chang&Cohen1978]] | | | pKO3, pKOV | Suc^S, Cam^R | | | [[http://www.ncbi.nlm.nih.gov/pubmed/9335267][Link1997]] | Church vectors for creating deletions; note: Grow at 30° Celsius | | pACBSR | Cam^R | p15A | | [[http://www.ncbi.nlm.nih.gov/pubmed/12853150][Herring2003]] | Low copy number. Use a midi or maxiprep. | | pRA300, pRA301, pRA302 | Spec^R | | | [[http://www.jbc.org/content/277/18/15773.full.pdf+html][Akakura2002]] | Broad host range, promoterless _lacZ_ [[%ATTACHURLPATH%/pRA_map.png][map]] | | pCP20 | Amp^R | TS | BT340 | [[ProcedureFLPFRTRecombination][FLP recombinase protocol]] [[http://msb.embopress.org/content/2/1/2006.0008.long][Baba 2006]] | Temperature sensitive origin, FLP recombinase. Commonly used for removal of KAN cassette from Keio collection. | | pMMB67EH | Amp^R | RSF1010 | | [[http://www.atcc.org/products/all/37622.aspx][ATCC 37622]] | Broad host range conjugative plasmid | Plasmids listed on this page reside in the common plasmid box in the –80°C freezers in both Welch and MBB labs. ---++ Useful Links * [[http://www.qiagen.com/knowledge-and-support/spotlight/plasmid-resource-center/growth%20of%20bacterial%20cultures][Origins of replication and copy numbers of various plasmids and cosmids - Qiagen]]

   

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Topic attachments

I	Attachment	History	Action	Size	Date	Who	Comment
gbk	pACYC184.gbk	r1	manage	7.7 K	2009-08-03 - 21:09	JeffreyBarrick
gbk	pCA24N.gbk	r1	manage	9.9 K	2009-05-29 - 13:21	JeffreyBarrick
gbk	pCR2.1.gbk	r2 r1	manage	7.1 K	2012-03-07 - 21:11	JeffreyBarrick
gbk	pKD13_Kan_template.gbk	r1	manage	7.6 K	2009-05-29 - 13:22	JeffreyBarrick
gbk	pKD3_Cam_template.gbk	r1	manage	6.6 K	2009-05-29 - 13:22	JeffreyBarrick
gb	pKD46.gb	r1	manage	16.4 K	2012-01-20 - 21:09	BrianRenda
txt	pKOV.txt	r1	manage	10.6 K	2011-02-21 - 17:13	JeffreyBarrick
gbk	pkd4.gbk	r1	manage	6.1 K	2011-12-19 - 22:29	BrianRenda
gbk	pkd4_with_gfp_sites_marked.gbk	r1	manage	10.0 K	2011-12-19 - 22:35	BrianRenda

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Contributors to this topic JeffreyBarrick, CraigBarnhart, DanielDeatherage, BrianRenda, AlvaroRodriguez

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