LOCUS AY048744 3434 bp DNA circular SYN 11-SEP-2001 DEFINITION Template plasmid pKD13, complete sequence. ACCESSION AY048744 VERSION AY048744.1 GI:15554335 KEYWORDS . SOURCE Template plasmid pKD13 ORGANISM Template plasmid pKD13 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3434) AUTHORS Datsenko,K.A. and Wanner,B.L. TITLE One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6640-6645 (2000) PUBMED 10829079 REFERENCE 2 (bases 1 to 3434) AUTHORS Datsenko,K.A. and Wanner,B.L. TITLE Direct Submission JOURNAL Submitted (29-JUL-2001) Biological Sciences, Purdue University, Lilly Hall of Life Sciences, West Lafayette, IN 47907, USA FEATURES Location/Qualifiers source 1..3434 /organism="Template plasmid pKD13" /mol_type="genomic DNA" /db_xref="taxon:170493" misc_feature 31..50 /note="priming site 1" misc_feature complement(51..98) /note="natural FRT site" CDS complement(111..905) /note="kanamycin resistance" /codon_start=1 /transl_table=11 /product="Tn5 neomycin phosphotransferase" /protein_id="AAL02037.1" /db_xref="GI:15554336" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" misc_feature 274..293 /note="common priming site kt" misc_feature 744..763 /note="common priming site k2" misc_feature 835..854 /note="common priming site k1" misc_feature complement(1273..1307) /note="distal 35-nt of natural FRT site" misc_feature 1334..1353 /note="priming site 4" terminator 1402..1644 /note="lambda terminator" rep_origin 1678..2076 /note="R6K gamma replication origin" CDS complement(2146..3006) /note="bla; ampicillin resistance" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AAL02038.1" /db_xref="GI:15554337" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" terminator 3153..3412 /note="rrnB transcription terminator" ORIGIN 1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta 61 tactttctag agaataggaa cttcggaata ggaacttcaa gatcccctta ttagaagaac 121 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc 181 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac 241 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag 301 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc 361 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga 421 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc 481 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc 541 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg 601 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg 661 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg 721 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc 781 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca 841 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca 901 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag 961 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag 1021 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat 1081 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc 1141 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc 1201 tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgagctt 1261 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcgaac tgcaggtcga 1321 cggatccccg gaattaattc tcatgtttga cagcttatca ctgatcagtg aattaatggc 1381 gatgacgcat cctcacgata atatccgggt aggcgcaatc actttcgtct ctactccgtt 1441 acaaagcgag gctgggtatt tcccggcctt tctgttatcc gaaatccact gaaagcacag 1501 cggctggctg aggagataaa taataaacga ggggctgtat gcacaaagca tcttctgttg 1561 agttaagaac gagtatcgag atggcacata gccttgctca aattggaatc aggtttgtgc 1621 caataccagt agaaacagac gaagaagcta gctttgcact ggattgcgag gctttgccat 1681 ggctaattcc catgtcagcc gttaagtgtt cctgtgtcac tgaaaattgc tttgagaggc 1741 tctaagggct tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt taaaccttaa 1801 aagctttaaa agccttatat attctttttt ttcttataaa acttaaaacc ttagaggcta 1861 tttaagttgc tgatttatat taattttatt gttcaaacat gagagcttag tacgtgaaac 1921 atgagagctt agtacgttag ccatgagagc ttagtacgtt agccatgagg gtttagttcg 1981 ttaaacatga gagcttagta cgttaaacat gagagcttag tacgtgaaac atgagagctt 2041 agtacgtact atcaacaggt tgaactgcgg atcttgcggc cgcaaaaatt aaaaatgaag 2101 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 2161 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 2221 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 2281 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 2341 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 2401 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 2461 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 2521 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 2581 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 2641 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 2701 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 2761 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 2821 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 2881 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 2941 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 3001 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 3061 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 3121 ccgaaaagtg ccacctgcat cgatggcccc ccgatggtag tgtggggtct ccccatgcga 3181 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 3241 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg 3301 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact 3361 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtggccagtg 3421 ccaagcttgc atgc //