Commonly Used Plasmids

plasmid	markers	origin	host	ref	description
pCA24N	Cam^R			Kitagawa2005	ASKA collection vector
pKD46	Amp^R	TS	?	Datsenko&Wanner2000	Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD78	Cam^R	TS			Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3	Cam^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R				TOPO cloning kit vector
pACYC184	Tet^R, Cam^R		ER2420	Chang&Cohen1978	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R			Link1997	Church vectors for creating deletions; note: Grow at 30° Celsius
pACBSR	Cam^R	p15A		Herring2003	Low copy number. Use a midi or maxiprep.
pRA300, pRA301, pRA302	Spec^R			Akakura2002	Broad host range, promoterless lacZ map
pCP20	Amp^R	TS	BT340	FLP recombinase protocol Baba 2006	Temperature sensitive origin, FLP recombinase. Commonly used for removal of KAN cassette from Keio collection.

Plasmids listed on this page reside in the common plasmid box in the –80°C freezers in both Welch and MBB labs.

Useful Links

Topic attachments
I	Attachment	History	Action	Size	Date	Who
gbk	pACYC184.gbk	r1	manage	7.7 K	2009-08-03 - 21:09	JeffreyBarrick
gbk	pCA24N.gbk	r1	manage	9.9 K	2009-05-29 - 13:21	JeffreyBarrick
gbk	pCR2.1.gbk	r2 r1	manage	7.1 K	2012-03-07 - 21:11	JeffreyBarrick
gbk	pKD13_Kan_template.gbk	r1	manage	7.6 K	2009-05-29 - 13:22	JeffreyBarrick
gbk	pKD3_Cam_template.gbk	r1	manage	6.6 K	2009-05-29 - 13:22	JeffreyBarrick
gb	pKD46.gb	r1	manage	16.4 K	2012-01-20 - 21:09	BrianRenda
txt	pKOV.txt	r1	manage	10.6 K	2011-02-21 - 17:13	JeffreyBarrick
gbk	pkd4.gbk	r1	manage	6.1 K	2011-12-19 - 22:29	BrianRenda
gbk	pkd4_with_gfp_sites_marked.gbk	r1	manage	10.0 K	2011-12-19 - 22:35	BrianRenda

Contributors to this topic

JeffreyBarrick, CraigBarnhart, BrianRenda, DanielDeatherage, AlvaroRodriguez

Topic revision: r22 - 2014-05-12 - 16:03:13 - Main.DanielDeatherage