LOCUS       AB052891                5240 bp    DNA     circular SYN 11-MAY-2005
DEFINITION  Cloning vector pCA24N DNA, complete genome.
ACCESSION   AB052891
VERSION     AB052891.2  GI:63147361
KEYWORDS    .
SOURCE      Cloning vector pCA24N
  ORGANISM  Cloning vector pCA24N
            other sequences; artificial sequences; vectors.
REFERENCE   1
  AUTHORS   Kitagawa,M.
  TITLE     Archive Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Kitagawa,M., Hirai,A. and Mori,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2000) Hirotada Mori, Nara Institute of Science &
            Technology, Research & Education Center of Genetic Information;
            Takayama 8916-5, Ikoma, Nara 630-0101, Japan
            (E-mail:hmori@gtc.naist.jp, Tel:81-743-72-5662, Fax:81-743-72-5669)
COMMENT     On May 10, 2005 this sequence version replaced gi:12082329.
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Cloning vector pCA24N"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:146876"
     promoter        71..163
                     /standard_name="p-T5-lac"
     gene            185..250
                     /gene="his-tag"
     CDS             185..250
                     /gene="his-tag"
                     /codon_start=1
                     /transl_table=11
                     /product="6xHIS"
                     /protein_id="BAB20664.1"
                     /db_xref="GI:12082330"
                     /translation="MRGSHHHHHHTDPALRPMRPQ"
     gene            <234..959
                     /gene="gcn4"
     misc_feature    <234..959
                     /gene="gcn4"
                     /product="GFPuv4"
     gene            1139..1320
                     /gene="rrnB-T1"
     terminator      1139..1320
                     /gene="rrnB-T1"
     gene            1761..2420
                     /gene="cat"
     CDS             1761..2420
                     /gene="cat"
                     /codon_start=1
                     /transl_table=11
                     /product="CAT"
                     /protein_id="BAB20666.1"
                     /db_xref="GI:12082332"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     rep_origin      3556
     gene            3879..4961
                     /gene="lacI^q"
     CDS             3879..4961
                     /gene="lacI^q"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI"
                     /protein_id="BAB20667.1"
                     /db_xref="GI:12082333"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
ORIGIN      
        1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
       61 tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat
      121 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt
      181 aactatgaga ggatctcacc atcaccatca ccatacggat ccggccctga ggcctatgcg
      241 gccgcagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      301 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      361 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      421 ttgtcactac tctgacgtat ggtgttcaat gcttttcccg ttatccggat catatgaaac
      481 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt
      541 tcaaagatga cgggaactac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg
      601 ttaatcgtat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt ctcggacaca
      661 aactcgagta caactataac tcacacaatg tatacatcac ggcagacaaa caaaagaatg
      721 gaatcaaagc taacttcaaa attcgccaca acattgaaga tggatccgtt caactagcag
      781 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      841 acctgtcgac acaatctgcc cttttgaaag atcccaacga aaagcgtgac cacatggtcc
      901 ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgaacta tacaaataag
      961 cggccgctaa gggtcgacct gcagccaagc ttaattagct gagcttggac tcctgttgat
     1021 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg
     1081 ggcgtttttt attggtgaga atccaagcta ggggaattca tcgtgactga ctgacgatct
     1141 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     1201 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     1261 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     1321 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1381 taatggtttc ttaggatcct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc
     1441 agtagctgaa caggagggac agctgataga aacagaagcc actggagcac ctcaaaaaca
     1501 ccatcataca ctaaatcagt aagttggcag catcacccga cgcactttgc gccgaataaa
     1561 tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg
     1621 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca
     1681 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
     1741 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     1801 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     1861 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     1921 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
     1981 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     2041 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     2101 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     2161 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     2221 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     2281 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     2341 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     2401 agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtg
     2461 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac
     2521 ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc
     2581 ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt
     2641 gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag
     2701 agcctgataa aaacggttag gatcggagtc aggcaactat ggatgaacga aatagacaga
     2761 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2821 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2881 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2941 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3001 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3061 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3121 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3181 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3241 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3301 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3361 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3421 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3481 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3541 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3601 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3661 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3721 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3781 tttcacaccg cataaattcc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat
     3841 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg
     3901 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
     3961 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     4021 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     4081 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     4141 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     4201 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     4261 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     4321 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     4381 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     4441 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     4501 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     4561 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     4621 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     4681 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     4741 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     4801 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     4861 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4921 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     4981 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     5041 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgga
     5101 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5161 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5221 tcgcccttcc caacagttgc
//

