LOCUS       pKD4    3267 bp    DNA    SYN    19-Dec-2011
DEFINITION  pKD4
ACCESSION    
KEYWORDS    
SOURCE    
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="pKD4"
                     /mol_type="other DNA"
     misc_feature    complement(51..98)
                     /label="FRT"
     promoter        321..370
                     /label="NEOKAN_promoter"
     CDS             459..1253
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /note="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            462..1250
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     rep_origin      1523..1894
                     /label="R6Kg_origin"
     misc_feature    1882..1900
                     /label="pUni_fwd_primer"
     CDS             complement(1979..2839)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /note="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     gene            complement(1979..2839)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     promoter        complement(2881..2909)
                     /label="AmpR_promoter"
     terminator      3038..3081
                     /label="rrnB_T1_terminator"
     terminator      3213..3240
                     /label="rrnB_T2_terminator"
ORIGIN
    1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta 
   61 tactttctag agaataggaa cttcggaata ggaacttcaa gatcccctca cgctgccgca 
  121 agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga 
  181 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa 
  241 gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg 
  301 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga 
  361 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat 
  421 caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 
  481 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 
  541 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 
  601 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 
  661 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 
  721 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 
  781 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 
  841 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 
  901 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 
  961 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 
 1021 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 
 1081 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 
 1141 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 
 1201 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 
 1261 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 
 1321 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 
 1381 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccagct tcaaaagcgc 
 1441 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaacta aggaggatat 
 1501 tcatatggac catggctaat tcccatgtca gccgttaagt gttcctgtgt cactgaaaat 
 1561 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac 
 1621 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa 
 1681 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct 
 1741 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg 
 1801 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga 
 1861 aacatgagag cttagtacgt actatcaaca ggttgaactg cggatcttgc ggccgcaaaa 
 1921 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
 1981 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
 2041 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
 2101 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
 2161 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
 2221 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
 2281 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
 2341 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
 2401 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
 2461 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
 2521 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
 2581 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
 2641 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
 2701 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
 2761 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
 2821 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
 2881 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
 2941 cgcgcacatt tccccgaaaa gtgccacctg catcgatggc cccccgatgg tagtgtgggg 
 3001 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa 
 3061 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa 
 3121 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg 
 3181 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt 
 3241 tgcgtggcca gtgccaagct tgcatgc
//