Barrick Lab :: ProtocolsRecipesM9Minimal

---+++ M9 Minimal Medium

|  *1L*  |   *Component*  |
| 6 g | Sodium phosphate dibasic (anhydrous), Na<sub>2</sub>HPO<sub>4</sub>  |  
| 3 g | Potassium phosphate monobasic, KH<sub>2</sub>PO<sub>4</sub> |
| 0.5 g | Sodium chloride, !NaCl |
| 1 g | Ammonium chloride, NH<sub>4</sub>Cl | 
Add dH<sub>2</sub>O to 1 liter. Autoclave.

After media is autoclaved add the following sterile ingredients:
|  *1L*  |   *Component*  |
| 1 ml | 1M Magnesium sulfate, !MgSO<sub>4</sub> |
| 1 ml | 0.1M Calcium chloride !CaCl<sub>2</sub> |
| 10 ml |10% (w/v) glucose (or other carbon source) |

| *Final composition* ||
| 93.0 mM | Sodium (Na<sup>+</sup>) |
| 22.1 mM | Potassium (K<sup>+</sup>) |
| 18.7 mM | Ammonium (NH<sub>4</sub>) | 
| 1.0 mM | Calcium (Ca<sup>2+</sup>) |
| 0.1 mM | Magnesium (Mg<sup>2+</sup>) |
| 29.2 mM | Chloride (Cl<sup>-</sup>|
| 0.1 mM | Sulfate (SO<sub>4</sub><sup>2-</sup>) |
| 42.2 mM | Phosphate (PO<sub>4</sub><sup>3-</sup>) |

Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesM9Minimal

Contributors to this topic

CraigBarnhart, JordanMonk, JeffreyBarrick, AurkoDasgupta

Topic revision: r8 - 2018-11-14 - 17:20:20 - Main.JeffreyBarrick