---+++ M9 Minimal Medium | *1L* | *Component* | | 6 g | Sodium phosphate dibasic (anhydrous), Na<sub>2</sub>HPO<sub>4</sub> | | 3 g | Potassium phosphate monobasic, KH<sub>2</sub>PO<sub>4</sub> | | 0.5 g | Sodium chloride, !NaCl | | 1 g | Ammonium chloride, NH<sub>4</sub>Cl | Add dH<sub>2</sub>O to 1 liter. Autoclave. After media is autoclaved add the following sterile ingredients: | *1L* | *Component* | | 1 ml | 1M Magnesium sulfate, !MgSO<sub>4</sub> | | 1 ml | 0.1M Calcium chloride !CaCl<sub>2</sub> | | 10 ml |10% (w/v) glucose (or other carbon source) | | *Final composition* || | 93.0 mM | Sodium (Na<sup>+</sup>) | | 22.1 mM | Potassium (K<sup>+</sup>) | | 18.7 mM | Ammonium (NH<sub>4</sub>) | | 1.0 mM | Calcium (Ca<sup>2+</sup>) | | 0.1 mM | Magnesium (Mg<sup>2+</sup>) | | 29.2 mM | Chloride (Cl<sup>-</sup>| | 0.1 mM | Sulfate (SO<sub>4</sub><sup>2-</sup>) | | 42.2 mM | Phosphate (PO<sub>4</sub><sup>3-</sup>) |
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Topic revision: r8 - 2018-11-14 - JeffreyBarrick