(r4) Barrick Lab :: ProtocolsRecipesM9Minimal

Make 5× M9 salts:

1L	Component
64 g	Sodium phosphate (dibasic, 7 hydrate) Na₂HPO₄•H₂O
15 g	Potassium phosphate (monobasic) KH₂PO₄
2.5 g	Sodium chloride, NaCl
5 g	Ammonium chloride NH₄Cl

Add dH₂O to 1 liter. Autoclave.

Combine the following ingredients (all pre-sterilized):

1L	Component
200 ml	5× M9 salts
2 ml	1M Magnesium sulfate MgSO₄
0.1 ml	1 M Calcium chloride CaCl₂
40 ml	10% (w/v) glucose (or other carbon source)

Add sterile dH₂O to 1 liter. Autoclave.

To make M9 plates add the items above to 16g of sterilized agar.
If making less than 1 liter use 8g of agar per 500ml of solution.

Contributors to this topic

CraigBarnhart, AurkoDasgupta, JeffreyBarrick, JordanMonk

Topic revision: r4 - 2011-07-29 - 14:37:29 - Main.CraigBarnhart