M9 Minimal Medium

1L Component
6 g Sodium phosphate dibasic (anhydrous), Na2HPO4
3 g Potassium phosphate monobasic, KH2PO4
0.5 g Sodium chloride, NaCl
1 g Ammonium chloride, NH4Cl
Add dH2O to 1 liter. Autoclave.

After media is autoclaved add the following sterile ingredients:

1L Component
1 ml 1M Magnesium sulfate, MgSO4
1 ml 0.1M Calcium chloride CaCl2
10 ml 10% (w/v) glucose (or other carbon source)

Final composition
93.0 mM Sodium (Na+)
22.1 mM Potassium (K+)
18.7 mM Ammonium (NH4)
1.0 mM Calcium (Ca2+)
0.1 mM Magnesium (Mg2+)
29.2 mM Chloride (Cl-
0.1 mM Sulfate (SO42-)
42.2 mM Phosphate (PO43-)
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