M9 Minimal Media Plates

As with DM and MG media, make sure to autoclave the agar and phosphate separately.

For 1 liter of media:

1L Component
6 g Sodium phosphate, Na2HPO4 (anhydrous)
3 g Potassium phosphate, KH2PO4
0.5 g Sodium chloride, NaCl
1 g Ammonium chloride, NH4Cl

Add dH2O to 500 mL

1L Component
16 g Agar
1 ml Antifoam

Add dH2O to 500 mL.

Autoclave media. Once the bottles are cooled to 55C, mix the agar and M9 components.

Next, add the following sterile ingredients:

1L Component
1 ml 1M Magnesium sulfate, MgSO4
1 ml 0.1M Calcium chloride CaCl2
10 ml 10% (w/v) glucose (or other carbon source)

 Barrick Lab  >  ProtocolsMediaRecipes  >  ProtocolsRecipesM9

Contributors to this topic edittopic DaciaLeon, CraigBarnhart
Topic revision: r3 - 27 Mar 2018 - 21:25:09 - Main.DaciaLeon
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