(r2) ProtocolsRecipesM9 < Lab

1L	Component
6 g	Sodium phosphate, Na₂HPO_{4 (anhydrous)}
3 g	Potassium phosphate, KH₂PO₄
0.5 g	Sodium chloride, NaCl
1 g	Ammonium chloride, NH₄Cl
16 g	Agar
1 ml	Antifoam

Add dH₂O to 1 liter. Autoclave.

After media is autoclaved add the following sterile ingredients:

1L	Component
1 ml	1M Magnesium sulfate, MgSO₄
1 ml	0.1M Calcium chloride CaCl₂
10 ml	10% (w/v) glucose (or other carbon source)

Topic revision: r2 - 2018-03-26 - 17:38:26 - Main.DaciaLeon