M9 Minimal Media Plates
As with DM and MG media, make sure to autoclave the agar and phosphate separately.
For 1 liter of media:
| 1L |
Component |
| 6 g |
Sodium phosphate, Na2HPO4 (anhydrous) |
| 3 g |
Potassium phosphate, KH2PO4 |
| 0.5 g |
Sodium chloride, NaCl |
| 1 g |
Ammonium chloride, NH4Cl |
Add dH
2O to
500 mL
| 1L |
Component |
| 16 g |
Agar |
| 1 ml |
Antifoam |
Add dH
2O to
500 mL.
Autoclave media. Once the bottles are cooled to 55°C, mix the agar and M9 components.
Next, add the following sterile ingredients:
| 1L |
Component |
| 1 ml |
1M Magnesium sulfate, MgSO4 |
| 1 ml |
0.1M Calcium chloride CaCl2 |
| 10 ml |
10% (w/v) glucose (or other carbon source) |
