Barrick Lab :: ProtocolsRecipesM9

---+++ M9 Minimal Media Plates

As with DM and MG media, make sure to autoclave the agar and phosphate separately.

For 1 liter of media:

|  *1L*  | *Component*  |
| 6 g | Sodium phosphate, Na<sub>2</sub>HPO<sub>4 (anhydrous)</sub>  |
| 3 g | Potassium phosphate, KH<sub>2</sub>PO<sub>4</sub> |
| 0.5 g | Sodium chloride, NaCl |
| 1 g | Ammonium chloride, NH<sub>4</sub>Cl |

Add dH<sub>2</sub>O to *500* mL

|  *1L*  | *Component*  |
| 16 g | Agar |
| 1 ml | Antifoam |

Add dH<sub>2</sub>O to *500* mL.

Autoclave media. Once the bottles are cooled to 55°C, mix the agar and M9 components.

Next, add the following sterile ingredients:

|  *1L*  | *Component*  |
| 1 ml | 1M Magnesium sulfate, MgSO<sub>4</sub> |
| 1 ml | 0.1M Calcium chloride CaCl<sub>2</sub> |
| 10 ml | 10% (w/v) glucose (or other carbon source)  |

Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesM9

Contributors to this topic

DaciaLeon, CraigBarnhart

Topic revision: r3 - 2018-03-27 - 21:25:09 - Main.DaciaLeon