ProtocolUpdatesNeeded < Lab

---+ Protocol Updates Needed
   * [[ProcedureDNASequencing][this page on DNA sequencing]] needs to be updated and be made more for the general public than to the lab
   * General guide for sorting bacteria. This information is not widely available and I had to consult r/flowcytometry to find out. (Tyler)
      * Things like performing a dilution vs eps calibration curve to avoid swarming, sample prep, etc.
   * Updating fluctuation test page to include better tools for calculating mutation rate (mlemur). (Tyler)
   * Add a page for purifying and storing HMW DNA (Isaac)
   * Update PCR protocol to emphasize not adding too much template (ex: miniprep). 
      * Ideally, also include a calculation of how many cycles are needed.
   * <del>Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?</del>
   * <del>Add a page with standard plate reader settings for different fluorophores (with citations)</del>
      * Also explain setting the gain!
   * Add a page describing tools for plotting and fitting growth curves
   * [[ProtocolsStandardPCR][Standard PCR]] – add troubleshooting information here?
   * Page with books/resources for students joining the lab? (Lucio)
   * Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
   * Nuke most topics under computation and many under reference (Dan)
   * Link all the golden gate materials (and work on general GGA materials)
   * PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
   * Migrate LTEE stuff to LTEE website (Jeff)
   * Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
   * <del>[[SoftwareList][Computation tool list]] -- Add pLannotate and OSTIR (Cameron)</del>
   * <del>[[LabPersonnel][Team]] – add start dates, be sure no former lab members were not transferred there.</del>
   * <del>Update research interests page (Jeff)</del>
   * <del>[[ProtocolsLearnBiocomputing][Anaconda should not be recommended]]</del>
   * Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
   * Add information about Lactobacillus strains in bees.
   * Troubleshooting minipreps, handling isolation of large plasmids
Barrick Lab > ProtocolList > ProtocolUpdatesNeeded
Topic revision: r42 - 2025-05-20 - 14:41:14 - Main.IraZibbu