Difference: ProtocolUpdatesNeeded (1 vs. 42)

Revision 422025-05-20 - IraZibbu

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • this page on DNA sequencing needs to be updated and be made more for the general public than to the lab
  • General guide for sorting bacteria. This information is not widely available and I had to consult r/flowcytometry to find out. (Tyler)
    • Things like performing a dilution vs eps calibration curve to avoid swarming, sample prep, etc.
  • Updating fluctuation test page to include better tools for calculating mutation rate (mlemur). (Tyler)
  • Add a page for purifying and storing HMW DNA (Isaac)
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep).
    • Ideally, also include a calculation of how many cycles are needed.
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Update research interests page (Jeff)
  • Anaconda should not be recommended
  • Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
  • Add information about Lactobacillus strains in bees.
Added:
>
>
  • Troubleshooting minipreps, handling isolation of large plasmids

Revision 412025-03-20 - CameronRoots

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Added:
>
>
 
  • General guide for sorting bacteria. This information is not widely available and I had to consult r/flowcytometry to find out. (Tyler)
    • Things like performing a dilution vs eps calibration curve to avoid swarming, sample prep, etc.
  • Updating fluctuation test page to include better tools for calculating mutation rate (mlemur). (Tyler)
  • Add a page for purifying and storing HMW DNA (Isaac)
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep).
    • Ideally, also include a calculation of how many cycles are needed.
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Update research interests page (Jeff)
  • Anaconda should not be recommended
  • Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
  • Add information about Lactobacillus strains in bees.

Revision 402025-03-20 - CameronRoots

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • General guide for sorting bacteria. This information is not widely available and I had to consult r/flowcytometry to find out. (Tyler)
    • Things like performing a dilution vs eps calibration curve to avoid swarming, sample prep, etc.
  • Updating fluctuation test page to include better tools for calculating mutation rate (mlemur). (Tyler)
  • Add a page for purifying and storing HMW DNA (Isaac)
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep).
    • Ideally, also include a calculation of how many cycles are needed.
Changed:
<
<
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
>
>
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
 
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Update research interests page (Jeff)
  • Anaconda should not be recommended
  • Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
  • Add information about Lactobacillus strains in bees.

Revision 392025-03-17 - TylerDeJong

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • General guide for sorting bacteria. This information is not widely available and I had to consult r/flowcytometry to find out. (Tyler)
Changed:
<
<
    • Things like performing a dilution vs eps curve to avoid swarming, sample prep, etc.
>
>
    • Things like performing a dilution vs eps calibration curve to avoid swarming, sample prep, etc.
Added:
>
>
  • Updating fluctuation test page to include better tools for calculating mutation rate (mlemur). (Tyler)
 
  • Add a page for purifying and storing HMW DNA (Isaac)
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep).
    • Ideally, also include a calculation of how many cycles are needed.
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Update research interests page (Jeff)
  • Anaconda should not be recommended
  • Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
  • Add information about Lactobacillus strains in bees.

Revision 382025-03-17 - TylerDeJong

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Changed:
<
<
  • General guide for sorting bacteria. This information is not widely available and I had to consult the Sith lords of r/flowcytometry to find out. (Tyler)
    • Things like performing a dilution vs eps curve to avoid swarming, sample prep, enrichment sorts, etc.
>
>
  • General guide for sorting bacteria. This information is not widely available and I had to consult r/flowcytometry to find out. (Tyler)
    • Things like performing a dilution vs eps curve to avoid swarming, sample prep, etc.
 
  • Add a page for purifying and storing HMW DNA (Isaac)
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep).
    • Ideally, also include a calculation of how many cycles are needed.
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Update research interests page (Jeff)
  • Anaconda should not be recommended
  • Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
  • Add information about Lactobacillus strains in bees.

Revision 372025-03-09 - TylerDeJong

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Changed:
<
<
  • Guide for sorting bacteria since this information isn't widely available (performing a dilution vs eps curve to avoid swarming, sample prep, enrichment sorts, etc). (Tyler)
>
>
  • General guide for sorting bacteria. This information is not widely available and I had to consult the Sith lords of r/flowcytometry to find out. (Tyler)
Added:
>
>
    • Things like performing a dilution vs eps curve to avoid swarming, sample prep, enrichment sorts, etc.
 
  • Add a page for purifying and storing HMW DNA (Isaac)
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep).
    • Ideally, also include a calculation of how many cycles are needed.
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Update research interests page (Jeff)
  • Anaconda should not be recommended
  • Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
  • Add information about Lactobacillus strains in bees.

Revision 362025-03-07 - TylerDeJong

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Changed:
<
<
  • Guide for sorting bacteria since this information isn't widely available (performing a dilution vs eps curve to avoid swarming, etc). (Tyler)
>
>
  • Guide for sorting bacteria since this information isn't widely available (performing a dilution vs eps curve to avoid swarming, sample prep, enrichment sorts, etc). (Tyler)
 
  • Add a page for purifying and storing HMW DNA (Isaac)
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep).
    • Ideally, also include a calculation of how many cycles are needed.
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Update research interests page (Jeff)
  • Anaconda should not be recommended
  • Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
  • Add information about Lactobacillus strains in bees.

Revision 352025-03-07 - IsaacGifford

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • Guide for sorting bacteria since this information isn't widely available (performing a dilution vs eps curve to avoid swarming, etc). (Tyler)
  • Add a page for purifying and storing HMW DNA (Isaac)
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep).
    • Ideally, also include a calculation of how many cycles are needed.
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
Deleted:
<
<
  • Merge gel electrophoresis content (Isaac)
 
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Update research interests page (Jeff)
  • Anaconda should not be recommended
  • Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
  • Add information about Lactobacillus strains in bees.

Revision 342025-03-07 - TylerDeJong

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Added:
>
>
  • Guide for sorting bacteria since this information isn't widely available (performing a dilution vs eps curve to avoid swarming, etc). (Tyler)
 
  • Add a page for purifying and storing HMW DNA (Isaac)
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep).
    • Ideally, also include a calculation of how many cycles are needed.
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • Merge gel electrophoresis content (Isaac)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Update research interests page (Jeff)
  • Anaconda should not be recommended
  • Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
  • Add information about Lactobacillus strains in bees.

Revision 332025-03-07 - ZuberiAshraf

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • Add a page for purifying and storing HMW DNA (Isaac)
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep).
    • Ideally, also include a calculation of how many cycles are needed.
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • Merge gel electrophoresis content (Isaac)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Update research interests page (Jeff)
  • Anaconda should not be recommended
Added:
>
>
  • Update page about S. alvi - Include the risks of culturing in liquid and ideal culture time(s).
  • Add information about Lactobacillus strains in bees.

Revision 322025-03-07 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Changed:
<
<
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep)
>
>
  • Add a page for purifying and storing HMW DNA (Isaac)
Added:
>
>
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep).
    • Ideally, also include a calculation of how many cycles are needed.
 
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • Merge gel electrophoresis content (Isaac)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Update research interests page (Jeff)
  • Anaconda should not be recommended

Revision 312025-03-06 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Added:
>
>
  • Update PCR protocol to emphasize not adding too much template (ex: miniprep)
  • Do we have a page describing how to measure transformation efficiency and frequency - and what they should be for normal comp cells?
 
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • Merge gel electrophoresis content (Isaac)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
Changed:
<
<
  • Improve conjugation materials (Kadena). Improve 'working with S Alvi' page. (Tyler)
>
>
  • Improve conjugation materials (Kadena). Improve 'working with S alvi' page. (Tyler)
Deleted:
<
<
 

Revision 302023-09-21 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Deleted:
<
<
 
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
Deleted:
<
<
 
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • Merge gel electrophoresis content (Isaac)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S Alvi' page. (Tyler)
Added:
>
>

Revision 292023-09-20 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Changed:
<
<
>
>
 
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Anaconda should not be recommended
  • Update research interests page (Jeff)
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
  • Link all the golden gate materials (and work on general GGA materials)
  • Merge gel electrophoresis content (Isaac)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S Alvi' page. (Tyler)

Revision 282023-09-20 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • Computation tool list -- Add pLannotate and OSTIR (Cameron)
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
Changed:
<
<
  • Team – add start dates, be sure no former lab members were not transferred there.
>
>
  • Team – add start dates, be sure no former lab members were not transferred there.
 
Changed:
<
<
  • Update research interests page (Jeff)
>
>
  • Update research interests page (Jeff)
 
  • Page with books/resources for students joining the lab? (Lucio)
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference (Dan)
Changed:
<
<		* Link all the golden gate materials (and work on general GGA materials) * Merge gel electrophoresis content (Isaac) * PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ) * Migrate LTEE stuff to LTEE website (Jeff) * Improve conjugation materials (Kadena). Improve 'working with S Alvi' page. (Tyler)
>
>
  • Link all the golden gate materials (and work on general GGA materials)
  • Merge gel electrophoresis content (Isaac)
  • PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ)
  • Migrate LTEE stuff to LTEE website (Jeff)
  • Improve conjugation materials (Kadena). Improve 'working with S Alvi' page. (Tyler)

Revision 272023-09-19 - CameronRoots

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Changed:
<
<
>
>
 
  • Add a page with standard plate reader settings for different fluorophores (with citations)
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Team – add start dates, be sure no former lab members were not transferred there.
  • Anaconda should not be recommended
Changed:
<
<
  • Update research interests page
  • Page with books/resources for students joining the lab?
>
>
  • Update research interests page (Jeff)
  • Page with books/resources for students joining the lab? (Lucio)
 
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
Changed:
<
<
  • Nuke most topics under computation and many under reference
>
>
  • Nuke most topics under computation and many under reference (Dan)
Added:
>
>		* Link all the golden gate materials (and work on general GGA materials) * Merge gel electrophoresis content (Isaac) * PCR Troubleshooting Materials (Anthony, Cameron, Jack, PJ) * Migrate LTEE stuff to LTEE website (Jeff) * Improve conjugation materials (Kadena). Improve 'working with S Alvi' page. (Tyler)

Revision 262023-09-19 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Changed:
<
<
>
>
  • Computation tool list -- Add pLannotate and OSTIR
  • Add a page with standard plate reader settings for different fluorophores (with citations)
Added:
>
>
    • Also explain setting the gain!
  • Add a page describing tools for plotting and fitting growth curves
  • Standard PCR – add troubleshooting information here?
  • Team – add start dates, be sure no former lab members were not transferred there.
 
Changed:
<
<
>
>
  • Update research interests page
  • Page with books/resources for students joining the lab?
Added:
>
>
  • Similar page for those wanting to prepare for iGEM participation – I'm getting questions about what new synthetic biologists should read...
  • Nuke most topics under computation and many under reference

Revision 252023-02-01 - DanielDeatherage

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Added:
>
>
 

Revision 242023-01-29 - DanielDeatherage

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Changed:
<
<
>
>
 

Revision 232020-04-09 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"
Changed:
<
<

Protocol Updates Needed (as of 2016_Dec_15)

>
>

Protocol Updates Needed

 
Changed:
<
<

Protocols needed or flagged for updates

>
>
Deleted:
<
<
  • Flow protocol updates (Dan and Dacia)
  • P1 transduction; FLP/FRT recombination (Dan)
  • Chemical Competent Cells and Zcomp cells(Colin and Peng?)
  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility. (<- Dan?)
  • ProtocolsQuartzy - Instructions for using Quartzy to mark orders received, request supply orders, and order supplies for the lab (<- Simon?)
  • NewLabMemberChecklist - EHS training course list is out of date, computer setup for coding is incomplete/fewer people use MacPorts recently, also combine all online accounts under one header? (<- Simon?)
  • Update Gibson Assembly protocol - add images for clarity and future-proof language (location)

Recently finished protocols (kept until next Hackathon)

  • Trash.LabProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions <- Included in "working with non-canonical amino acids" section
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells. <- I updated EC protocol to include a large batch option, and split the transformation to a new page. Could still use pictures on both protocols.
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid. (finished!)
  • AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab (No longer relevant!)
  • ProtocolsncAAGFPIncorporationAssay - General instructions for a basic GFP Incorporation assay that can measure whether a particular synthetase pair incorporates a ncAA (we wrote a paper about this....)
  • LabMemberContactInformation - Lab members who left years ago still on list, newer lab members don't have most info added (mostly up to date)

Revision 222018-10-25 - SimonDAlton

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed (as of 2016_Dec_15)

Protocols needed or flagged for updates

  • Flow protocol updates (Dan and Dacia)
  • P1 transduction; FLP/FRT recombination (Dan)
  • Chemical Competent Cells and Zcomp cells(Colin and Peng?)
  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility. (<- Dan?)
  • ProtocolsQuartzy - Instructions for using Quartzy to mark orders received, request supply orders, and order supplies for the lab (<- Simon?)
  • NewLabMemberChecklist - EHS training course list is out of date, computer setup for coding is incomplete/fewer people use MacPorts recently, also combine all online accounts under one header? (<- Simon?)
  • Update Gibson Assembly protocol - add images for clarity and future-proof language (location)

Recently finished protocols (kept until next Hackathon)

Changed:
<
<
  • ProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions <- Included in "working with non-canonical amino acids" section
>
>
  • Trash.LabProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions <- Included in "working with non-canonical amino acids" section
 
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells. <- I updated EC protocol to include a large batch option, and split the transformation to a new page. Could still use pictures on both protocols.
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid. (finished!)
  • AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab (No longer relevant!)
  • ProtocolsncAAGFPIncorporationAssay - General instructions for a basic GFP Incorporation assay that can measure whether a particular synthetase pair incorporates a ncAA (we wrote a paper about this....)
  • LabMemberContactInformation - Lab members who left years ago still on list, newer lab members don't have most info added (mostly up to date)

Revision 212016-12-15 - SeanLeonard

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed (as of 2016_Dec_15)

Protocols needed or flagged for updates

  • Flow protocol updates (Dan and Dacia)
  • P1 transduction; FLP/FRT recombination (Dan)
  • Chemical Competent Cells and Zcomp cells(Colin and Peng?)
  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility. (<- Dan?)
Changed:
<
<
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
  • ProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions
>
>
  • ProtocolsQuartzy - Instructions for using Quartzy to mark orders received, request supply orders, and order supplies for the lab (<- Simon?)
  • NewLabMemberChecklist - EHS training course list is out of date, computer setup for coding is incomplete/fewer people use MacPorts recently, also combine all online accounts under one header? (<- Simon?)
Deleted:
<
<
  • ProtocolsQuartzy - Instructions for using Quartzy to mark orders received, request supply orders, and order supplies for the lab
  • NewLabMemberChecklist - EHS training course list is out of date, computer setup for coding is incomplete/fewer people use MacPorts recently, also combine all online accounts under one header?
 
  • Update Gibson Assembly protocol - add images for clarity and future-proof language (location)

Recently finished protocols (kept until next Hackathon)

Added:
>
>
  • ProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions <- Included in "working with non-canonical amino acids" section
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells. <- I updated EC protocol to include a large batch option, and split the transformation to a new page. Could still use pictures on both protocols.
 
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid. (finished!)
  • AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab (No longer relevant!)
  • ProtocolsncAAGFPIncorporationAssay - General instructions for a basic GFP Incorporation assay that can measure whether a particular synthetase pair incorporates a ncAA (we wrote a paper about this....)
  • LabMemberContactInformation - Lab members who left years ago still on list, newer lab members don't have most info added (mostly up to date)

Revision 202016-12-15 - SimonDOelsnitz

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed (as of 2016_Dec_15)

Protocols needed or flagged for updates

  • Flow protocol updates (Dan and Dacia)
  • P1 transduction; FLP/FRT recombination (Dan)
  • Chemical Competent Cells and Zcomp cells(Colin and Peng?)
  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility. (<- Dan?)
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
  • ProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions
  • ProtocolsQuartzy - Instructions for using Quartzy to mark orders received, request supply orders, and order supplies for the lab
  • NewLabMemberChecklist - EHS training course list is out of date, computer setup for coding is incomplete/fewer people use MacPorts recently, also combine all online accounts under one header?
Added:
>
>
  • Update Gibson Assembly protocol - add images for clarity and future-proof language (location)
 

Recently finished protocols (kept until next Hackathon)

  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid. (finished!)
  • AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab (No longer relevant!)
  • ProtocolsncAAGFPIncorporationAssay - General instructions for a basic GFP Incorporation assay that can measure whether a particular synthetase pair incorporates a ncAA (we wrote a paper about this....)
  • LabMemberContactInformation - Lab members who left years ago still on list, newer lab members don't have most info added (mostly up to date)

Revision 192016-12-15 - SeanLeonard

 
META TOPICPARENT name="ProtocolList"
Changed:
<
<

Protocol Updates Needed

>
>

Protocol Updates Needed (as of 2016_Dec_15)

Deleted:
<
<

Protocols and timeframes for updates:

 
Changed:
<
<

Protocols flagged pre-Wiki hackathon conception

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid.
  • AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab
>
>

Protocols needed or flagged for updates

  • Flow protocol updates (Dan and Dacia)
  • P1 transduction; FLP/FRT recombination (Dan)
  • Chemical Competent Cells and Zcomp cells(Colin and Peng?)
  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility. (<- Dan?)
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
 
  • ProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions
Deleted:
<
<
  • ProtocolsncAAGFPIncorporationAssay - General instructions for a basic GFP Incorporation assay that can measure whether a particular synthetase pair incorporates a ncAA
 
  • ProtocolsQuartzy - Instructions for using Quartzy to mark orders received, request supply orders, and order supplies for the lab
  • NewLabMemberChecklist - EHS training course list is out of date, computer setup for coding is incomplete/fewer people use MacPorts recently, also combine all online accounts under one header?
Changed:
<
<
  • LabMemberContactInformation - Lab members who left years ago still on list, newer lab members don't have most info added
>
>
Added:
>
>

Recently finished protocols (kept until next Hackathon)

  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid. (finished!)
  • AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab (No longer relevant!)
  • ProtocolsncAAGFPIncorporationAssay - General instructions for a basic GFP Incorporation assay that can measure whether a particular synthetase pair incorporates a ncAA (we wrote a paper about this....)
  • LabMemberContactInformation - Lab members who left years ago still on list, newer lab members don't have most info added (mostly up to date)

Revision 182016-04-04 - DanielDeatherage

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Added:
>
>

Protocols and timeframes for updates:

Protocols flagged pre-Wiki hackathon conception

 
  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid.
  • AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab
  • ProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions
  • ProtocolsncAAGFPIncorporationAssay - General instructions for a basic GFP Incorporation assay that can measure whether a particular synthetase pair incorporates a ncAA
  • ProtocolsQuartzy - Instructions for using Quartzy to mark orders received, request supply orders, and order supplies for the lab
  • NewLabMemberChecklist - EHS training course list is out of date, computer setup for coding is incomplete/fewer people use MacPorts recently, also combine all online accounts under one header?
  • LabMemberContactInformation - Lab members who left years ago still on list, newer lab members don't have most info added

Revision 172015-08-29 - JordanMonk

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid.
  • AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab
  • ProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions
  • ProtocolsncAAGFPIncorporationAssay - General instructions for a basic GFP Incorporation assay that can measure whether a particular synthetase pair incorporates a ncAA
  • ProtocolsQuartzy - Instructions for using Quartzy to mark orders received, request supply orders, and order supplies for the lab
  • NewLabMemberChecklist - EHS training course list is out of date, computer setup for coding is incomplete/fewer people use MacPorts recently, also combine all online accounts under one header?
Added:
>
>
  • LabMemberContactInformation - Lab members who left years ago still on list, newer lab members don't have most info added

Revision 162015-08-28 - JordanMonk

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid.
  • AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab
  • ProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions
  • ProtocolsncAAGFPIncorporationAssay - General instructions for a basic GFP Incorporation assay that can measure whether a particular synthetase pair incorporates a ncAA
Added:
>
>
  • ProtocolsQuartzy - Instructions for using Quartzy to mark orders received, request supply orders, and order supplies for the lab
  • NewLabMemberChecklist - EHS training course list is out of date, computer setup for coding is incomplete/fewer people use MacPorts recently, also combine all online accounts under one header?

Revision 152014-07-23 - SeanLeonard

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid.
  • AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab
Added:
>
>
  • ProtocolsncAAWorkingSolutions - Table of non-canonical amino acids (ncAAs) the lab has used and concentration/how to make stock solutions
  • ProtocolsncAAGFPIncorporationAssay - General instructions for a basic GFP Incorporation assay that can measure whether a particular synthetase pair incorporates a ncAA

Revision 142014-05-23 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
Deleted:
<
<
  • ProceduresAutoclavingDishwashing - (see bottom of protocol page) procedure for using autclaves, and various locations.
 
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid.
  • AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab

Revision 132013-09-03 - AlvaroRodriguez

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProceduresAutoclavingDishwashing - (see bottom of protocol page) procedure for using autclaves, and various locations.
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid.
Added:
>
>
  • AmountofLabStocks - (recommend revision of topic) Need to make a list for MBB lab and one for the Welch Lab

Revision 122013-08-20 - AurkoDasgupta

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProceduresAutoclavingDishwashing - (see bottom of protocol page) procedure for using autclaves, and various locations.
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
Deleted:
<
<
  • ProtocolsStandardPCR - needs a description of the polymerases available in lab and when to use each one.
 
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid.

Revision 112012-08-15 - CraigBarnhart

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProceduresAutoclavingDishwashing - (see bottom of protocol page) procedure for using autclaves, and various locations.
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
  • ProtocolsStandardPCR - needs a description of the polymerases available in lab and when to use each one.
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid.

Revision 102011-10-24 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProceduresAutoclavingDishwashing - (see bottom of protocol page) procedure for using autclaves, and various locations.
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
  • ProtocolsStandardPCR - needs a description of the polymerases available in lab and when to use each one.
Added:
>
>
  • ProtocolsDpnIDigestion - need instructions for performing DpnI digestion to remove template plasmid.

Revision 92011-10-05 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProceduresAutoclavingDishwashing - (see bottom of protocol page) procedure for using autclaves, and various locations.
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.
Added:
>
>
  • ProtocolsStandardPCR - needs a description of the polymerases available in lab and when to use each one.

Revision 82011-09-26 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProceduresAutoclavingDishwashing - (see bottom of protocol page) procedure for using autclaves, and various locations.
Changed:
<
<
  • ProceduresPrimerDatabase - instructions for adding primers to the lab database.
>
>
  • ProtocolsElectrocompetentCells - needs instructions for electroporating using our equipment and for creating larger batches of commonly used cells.

Revision 72011-09-26 - CraigBarnhart

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
Changed:
<
<
  • ProceduresAutoclavingDishwashing - procedure for using autclaves, and various locations.
>
>
  • ProceduresAutoclavingDishwashing - (see bottom of protocol page) procedure for using autclaves, and various locations.
 
  • ProceduresPrimerDatabase - instructions for adding primers to the lab database.

Revision 62011-06-20 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
Added:
>
>
  • ProceduresAutoclavingDishwashing - procedure for using autclaves, and various locations.
  • ProceduresPrimerDatabase - instructions for adding primers to the lab database.

Revision 52011-06-19 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Deleted:
<
<
  • ProceduresStandardAgaroseGel - new protocol for running an Agarose gel.
  • ProceduresPrimerDesign - needs complete overhaul.
 
  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
Deleted:
<
<
  • ProtocolsFreezingStrains - need new protocol for how to freeze.

Revision 42011-06-17 - AurkoDasgupta

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Changed:
<
<
  • ProtocolsAgaroseGel - new protocol for running an Agarose gel.
>
>
  • ProceduresStandardAgaroseGel - new protocol for running an Agarose gel.
 
  • ProceduresPrimerDesign - needs complete overhaul.
  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
Changed:
<
<
  • ProtocolsFreezerStocks - need new protocol for how to freeze.
>
>
  • ProtocolsFreezingStrains - need new protocol for how to freeze.

Revision 32011-06-15 - LindseyWolf

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

Deleted:
<
<
  • ProtocolsReagentRecipes - add recipe for "SB" gel running buffer.
 
  • ProtocolsAgaroseGel - new protocol for running an Agarose gel.
  • ProceduresPrimerDesign - needs complete overhaul.
  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProtocolsFreezerStocks - need new protocol for how to freeze.

Revision 22011-05-31 - CraigBarnhart

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProtocolsReagentRecipes - add recipe for "SB" gel running buffer.
  • ProtocolsAgaroseGel - new protocol for running an Agarose gel.
  • ProceduresPrimerDesign - needs complete overhaul.
  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
Changed:
<
<
  • ProtocolsFreezerStocks - need new protocol for how to freeze, label, and enter samples into database.
>
>
  • ProtocolsFreezerStocks - need new protocol for how to freeze.

Revision 12011-05-30 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Protocol Updates Needed

  • ProtocolsReagentRecipes - add recipe for "SB" gel running buffer.
  • ProtocolsAgaroseGel - new protocol for running an Agarose gel.
  • ProceduresPrimerDesign - needs complete overhaul.
  • ProceduresTargetedSequencing - need to update instructions for our kits and sequencing facility.
  • ProtocolsFreezerStocks - need new protocol for how to freeze, label, and enter samples into database.
View topic | History: r42 < r41 < r40 < r39 | More topic actions...
 
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