Commonly Used Plasmids

plasmid markers origin ref description
pCA24N CamR   Kitagawa2005 ASKA collection vector
pKD46 AmpR TS Datsenko&Wanner2000 Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD78 CamR TS   Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3 CamR   Datsenko&Wanner2000 Wanner template plasmid
pKD13 KanR   Datsenko&Wanner2000 Wanner template plasmid
pCR2.1 KanR, AmpR     TOPO cloning kit vector
pACYC184 TetR, CamR   Chang&Cohen1978 Lenski evolution experiment
pKO3, pKOV SucS, CamR   Link1997 Church vectors for creating deletions; note: Grow at 30 degrees Celsius
pRA300, pRA301, pRA302 SpecR   Akakura2002 Broad host range, promoterless lacZ map

Common Plasmid Box

Copy of box resides in –80°C freezer in both Welch and MBB.

plasmid strain
pACBSR TOP10
pKD3 BW25141
pKD13 BW2514
pKD32 BW2514
pKD46 BW25141
pCP20 BT340
pRA300 ?
pRA301 ?
pRA302 ?
pACYC184 ER2420

References

Akakura2002 Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.
Chang&Cohen1978 Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156.
Datsenko&Wanner2000 Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):6640–6645.
Kitagawa2005 Kitagawa, M. et al. (2005) DNA Res. 12: 291-9
Link1997 Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37.
Attachments Attachments
Topic attachments
I Attachment History Action Size Date Who Comment
Unknown file formatgbk pACYC184.gbk r1 manage 7.7 K 2009-08-03 - 21:09 JeffreyBarrick  
Unknown file formatgbk pCA24N.gbk r1 manage 9.9 K 2009-05-29 - 13:21 JeffreyBarrick  
Unknown file formatgbk pCR2.1.gbk r2 r1 manage 7.1 K 2012-03-07 - 21:11 JeffreyBarrick  
Unknown file formatgbk pKD13_Kan_template.gbk r1 manage 7.6 K 2009-05-29 - 13:22 JeffreyBarrick  
Unknown file formatgbk pKD3_Cam_template.gbk r1 manage 6.6 K 2009-05-29 - 13:22 JeffreyBarrick  
Unknown file formatgb pKD46.gb r1 manage 16.4 K 2012-01-20 - 21:09 BrianRenda  
Texttxt pKOV.txt r1 manage 10.6 K 2011-02-21 - 17:13 JeffreyBarrick  
Unknown file formatgbk pkd4.gbk r1 manage 6.1 K 2011-12-19 - 22:29 BrianRenda  
Unknown file formatgbk pkd4_with_gfp_sites_marked.gbk r1 manage 10.0 K 2011-12-19 - 22:35 BrianRenda  
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Contributors to this topic Edit topic JeffreyBarrick, CraigBarnhart, DanielDeatherage, BrianRenda, AlvaroRodriguez
Topic revision: r19 - 2013-03-25 - 18:43:30 - Main.DanielDeatherage
 
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