Commonly Used Plasmids

plasmid	markers	description	origin	instructions	reference
pCA24N	CamR	ASKA collection vector
pKD46	AmpR	Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3	CamR	Wanner template plasmid
pKD13	KanR	Wanner template plasmid
pCR2.1	KanR, AmpR	TOPO cloning kit vector
pACYC184	TetR, CamR	Lenski evolution experiment
pKO3, pKOV	SucS, CamR	Church vectors for creating deletions

Attachments Attachments

Topic attachments

I	Attachment	History	Action	Size	Date	Who	Comment
gbk	pACYC184.gbk	r1	manage	7.7 K	2009-08-03 - 21:09	JeffreyBarrick
gbk	pCA24N.gbk	r1	manage	9.9 K	2009-05-29 - 13:21	JeffreyBarrick
gbk	pCR2.1.gbk	r2 r1	manage	7.1 K	2012-03-07 - 21:11	JeffreyBarrick
gbk	pKD13_Kan_template.gbk	r1	manage	7.6 K	2009-05-29 - 13:22	JeffreyBarrick
gbk	pKD3_Cam_template.gbk	r1	manage	6.6 K	2009-05-29 - 13:22	JeffreyBarrick
gb	pKD46.gb	r1	manage	16.4 K	2012-01-20 - 21:09	BrianRenda	lambda red plasmid genbank file
txt	pKOV.txt	r1	manage	10.6 K	2011-02-21 - 17:13	JeffreyBarrick
gbk	pkd4.gbk	r1	manage	6.1 K	2011-12-19 - 22:29	BrianRenda
gbk	pkd4_with_gfp_sites_marked.gbk	r1	manage	10.0 K	2011-12-19 - 22:35	BrianRenda

 Barrick Lab  >  ReferenceList  >  ReferencePlasmids

Contributors to this topic JeffreyBarrick, CraigBarnhart, BrianRenda, DanielDeatherage, AlvaroRodriguez

• Barrick Lab
• Contact
• Research
• Publications
• Team
• Protocols
• Reference
• Software

• UT Austin
•    Mol Biosciences
•    ILS
•    Microbiology
•    EEB
•    CSSB
•    CBRS

• The LTEE
• iGEM team
• SynBioCyc
• SynBio course
• NGS course
• BEACON

• Search

• Log in