(r4) Barrick Lab :: ProtocolsRecipesSOB

---+++ SOB/SOC: Super Optimal Broth

*For SOB:* <br>
%TABLE{}%
|  *1L*  |  *Final [ ]*  |  *Component*  |
|  5 g   | 0.5% | yeast extract | 
|  20 g   | 2.0% | tryptone | 
| 0.5 g | 10 mM | NaCl |
| 0.186 g | 2 mM | KCl |
| 2.4 g | 20 mM | MgSO<sub>4</sub> (anhydrous) |
Add 800 ml of dH<sub>2</sub>O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH<sub>2</sub>O to a final volume 1 L. Autoclave

*For SOC (=SOB+glucose)*:<br>
Make SOB, except add dH<sub>2</sub>O to a final volume of 960 ml instead of 1 L.  Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% (w/v) glucose. (That's a final concentration of 0.4% glucose.)

*Note:* Some recipes for SOB use 10 mM MgSO<sub>4</sub> and 10 mM MgCl.

Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesSOB

Contributors to this topic

SophiaLin, JeffreyBarrick, AlexaMorton, AlvaroRodriguez, CraigBarnhart, KateElston

Topic revision: r4 - 2018-12-23 - 18:47:11 - Main.JeffreyBarrick