Difference: ProtocolsRecipesSOB (1 vs. 10)

Revision 102025-07-03 - AlexaMorton

 
META TOPICPARENT name="ProtocolsMediaRecipes"

SOB/SOC: Super Optimal Broth

For SOB:

200mL 250mL 1L Final [ ] Component
1 g 1.25 g 5 g 0.5% yeast extract
4 g 5 g 20 g 2.0% tryptone
0.12 g 0.125 g 0.5 g 10 mM NaCl
0.037 g* 0.042 g* 0.186 g 2 mM KCl
0.48 g 0.6 g 2.4 g 20 mM MgSO4 (anhydrous)

*Don't measure; it's just a pinch.

Adjust to pH 7.5 with 1M NaOH.

Changed:
<
<		Note: 200 ml of SOC makes 25 vials of 8 ml each.
>
>		Note: 200 ml of SOC makes 25 two-dram vials of 8 ml each.
  For 1 L: Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L.

Autoclave.

For SOC (=SOB+glucose)

Bulk scale: Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% (w/v) glucose. (That's a final concentration of 0.4% glucose or about 20 mM.)

Smaller scale: To achieve a 20 mM concentration of glucose for a known final volume n of SOC, you can calculate the amount of 10% w/v glucose to be added as 0.036n. The amount of SOB to add is then 0.964n. Keep units constant, so if n is in µL, the final amounts will also be in µL and so on.

  • Note: If you have a known volume of SOB x and want to know how much 10% w/v glucose to add to make SOC containing 20 mM glucose, simply divide x/0.964 to get your final volume n and subtract x from n to get the volume of 10% w/v glucose to add.

Generally when making stocks of SOC it is advisable to make smaller volume aliquots rather than larger ones. SOC media is extremely rich and can contaminate easily particularly after glucose is added. Additionally, smaller volumes tend to be pulled from it repeatedly with pipettes, which increases the chances of contamination over time.

Note: Some recipes for SOB use 10 mM MgSO4 and 10 mM MgCl.

Added:
>
>

Containers

Autoclave the two-dram vials of SOC in the plastic container and store in the cardboard container when dry. Make sure lids are unscrewed prior to autoclaving and screwed shut afterward. The plastic container can have the rack popped out (see image on the right) after autoclaving to dry any water that accumulated at the bottom of the box and avoid the growth of mold and such.

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Revision 92024-09-24 - AlexaMorton

 
META TOPICPARENT name="ProtocolsMediaRecipes"

SOB/SOC: Super Optimal Broth

For SOB:

200mL 250mL 1L Final [ ] Component
1 g 1.25 g 5 g 0.5% yeast extract
4 g 5 g 20 g 2.0% tryptone
0.12 g 0.125 g 0.5 g 10 mM NaCl
0.037 g* 0.042 g* 0.186 g 2 mM KCl
0.48 g 0.6 g 2.4 g 20 mM MgSO4 (anhydrous)

*Don't measure; it's just a pinch.

Adjust to pH 7.5 with 1M NaOH.

Note: 200 ml of SOC makes 25 vials of 8 ml each.

For 1 L: Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L.

Autoclave.

Changed:
<
<		For SOC (=SOB+glucose):
Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% (w/v) glucose. (That's a final concentration of 0.4% glucose.)
>
>

For SOC (=SOB+glucose)

Bulk scale: Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% (w/v) glucose. (That's a final concentration of 0.4% glucose or about 20 mM.)
 
Changed:
<
<		Generally when making stocks of SOC it is advisable to make up multiple 125 of 250ml bottles. This media is extremely rich and can contaminate easily (smaller volumes tend to be pulled from it repeatedly with pipettes, which increases the chances of contamination over time).
>
>		Smaller scale: To achieve a 20 mM concentration of glucose for a known final volume n of SOC, you can calculate the amount of 10% w/v glucose to be added as 0.036n. The amount of SOB to add is then 0.964n. Keep units constant, so if n is in µL, the final amounts will also be in µL and so on.
Added:
>
>
  • Note: If you have a known volume of SOB x and want to know how much 10% w/v glucose to add to make SOC containing 20 mM glucose, simply divide x/0.964 to get your final volume n and subtract x from n to get the volume of 10% w/v glucose to add.

Generally when making stocks of SOC it is advisable to make smaller volume aliquots rather than larger ones. SOC media is extremely rich and can contaminate easily particularly after glucose is added. Additionally, smaller volumes tend to be pulled from it repeatedly with pipettes, which increases the chances of contamination over time.

  Note: Some recipes for SOB use 10 mM MgSO4 and 10 mM MgCl.

Revision 82024-09-19 - SophiaLin

 
META TOPICPARENT name="ProtocolsMediaRecipes"

SOB/SOC: Super Optimal Broth

For SOB:

Changed:
<
<
250mL 1L Final [ ] Component
1.25 g 5 g 0.5% yeast extract
5 g 20 g 2.0% tryptone
0.125 g 0.5 g 10 mM NaCl
0.042 g* 0.186 g 2 mM KCl
0.6 g 2.4 g 20 mM MgSO4 (anhydrous)
>
>
200mL 250mL 1L Final [ ] Component
1 g 1.25 g 5 g 0.5% yeast extract
4 g 5 g 20 g 2.0% tryptone
0.12 g 0.125 g 0.5 g 10 mM NaCl
0.037 g* 0.042 g* 0.186 g 2 mM KCl
0.48 g 0.6 g 2.4 g 20 mM MgSO4 (anhydrous)
  *Don't measure; it's just a pinch.

Adjust to pH 7.5 with 1M NaOH.

Changed:
<
<		Note: 250 ml of SOC makes 25 vials of 10 ml each.
>
>		Note: 200 ml of SOC makes 25 vials of 8 ml each.
  For 1 L: Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L.

Autoclave.

For SOC (=SOB+glucose):
Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% (w/v) glucose. (That's a final concentration of 0.4% glucose.)

Deleted:
<
<		 For the 10 ml vials, add 200 µl of sterile 10% glucose.
  Generally when making stocks of SOC it is advisable to make up multiple 125 of 250ml bottles. This media is extremely rich and can contaminate easily (smaller volumes tend to be pulled from it repeatedly with pipettes, which increases the chances of contamination over time).

Note: Some recipes for SOB use 10 mM MgSO4 and 10 mM MgCl.

Revision 72024-09-10 - SophiaLin

 
META TOPICPARENT name="ProtocolsMediaRecipes"

SOB/SOC: Super Optimal Broth

For SOB:

Changed:
<
<
1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
2.4 g 20 mM MgSO4 (anhydrous)
>
>
250mL 1L Final [ ] Component
1.25 g 5 g 0.5% yeast extract
5 g 20 g 2.0% tryptone
0.125 g 0.5 g 10 mM NaCl
0.042 g* 0.186 g 2 mM KCl
0.6 g 2.4 g 20 mM MgSO4 (anhydrous)
Deleted:
<
<		For 1 L: Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L. Autoclave
 
Added:
>
>		*Don't measure; it's just a pinch.

Adjust to pH 7.5 with 1M NaOH.

Note: 250 ml of SOC makes 25 vials of 10 ml each.

For 1 L: Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L.

Autoclave.

 For SOC (=SOB+glucose):
Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% (w/v) glucose. (That's a final concentration of 0.4% glucose.)
Added:
>
>		 For the 10 ml vials, add 200 µl of sterile 10% glucose.
  Generally when making stocks of SOC it is advisable to make up multiple 125 of 250ml bottles. This media is extremely rich and can contaminate easily (smaller volumes tend to be pulled from it repeatedly with pipettes, which increases the chances of contamination over time).

Note: Some recipes for SOB use 10 mM MgSO4 and 10 mM MgCl.

Revision 62024-09-05 - SophiaLin

 
META TOPICPARENT name="ProtocolsMediaRecipes"

SOB/SOC: Super Optimal Broth

For SOB:

1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
2.4 g 20 mM MgSO4 (anhydrous)
Changed:
<
<		Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L. Autoclave
>
>		For 1 L: Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L. Autoclave
  For SOC (=SOB+glucose):
Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% (w/v) glucose. (That's a final concentration of 0.4% glucose.)

Generally when making stocks of SOC it is advisable to make up multiple 125 of 250ml bottles. This media is extremely rich and can contaminate easily (smaller volumes tend to be pulled from it repeatedly with pipettes, which increases the chances of contamination over time).

Note: Some recipes for SOB use 10 mM MgSO4 and 10 mM MgCl.

Revision 52021-07-08 - KateElston

 
META TOPICPARENT name="ProtocolsMediaRecipes"

SOB/SOC: Super Optimal Broth

For SOB:

1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
2.4 g 20 mM MgSO4 (anhydrous)
Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L. Autoclave

For SOC (=SOB+glucose):
Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% (w/v) glucose. (That's a final concentration of 0.4% glucose.)

Added:
>
>		 Generally when making stocks of SOC it is advisable to make up multiple 125 of 250ml bottles. This media is extremely rich and can contaminate easily (smaller volumes tend to be pulled from it repeatedly with pipettes, which increases the chances of contamination over time).
  Note: Some recipes for SOB use 10 mM MgSO4 and 10 mM MgCl.

Revision 42018-12-23 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

SOB/SOC: Super Optimal Broth

For SOB:

1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
2.4 g 20 mM MgSO4 (anhydrous)
Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L. Autoclave

For SOC (=SOB+glucose):

Changed:
<
<		Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% glucose.
>
>		Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% (w/v) glucose. (That's a final concentration of 0.4% glucose.)
  Note: Some recipes for SOB use 10 mM MgSO4 and 10 mM MgCl.

Revision 32018-12-10 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"
Changed:
<
<

SOB: Super Optimal Broth

>
>

SOB/SOC: Super Optimal Broth

 
Changed:
<
<
>
>		For SOB:
Deleted:
<
<
 
1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
Changed:
<
<
2.4 g 20 mM MgSO4
>
>
2.4 g 20 mM MgSO4 (anhydrous)
Added:
>
>		Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L. Autoclave
 
Changed:
<
<		Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume of 960 ml. Autoclave.
>
>		For SOC (=SOB+glucose):
Added:
>
>		Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% glucose.

Note: Some recipes for SOB use 10 mM MgSO4 and 10 mM MgCl.

 

Revision 22013-09-03 - AlvaroRodriguez

 
META TOPICPARENT name="ProtocolsMediaRecipes"

SOB: Super Optimal Broth

1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
2.4 g 20 mM MgSO4
Changed:
<
<		Add 800 ml of water and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add H2O to a final volume of 960 ml. Autoclave.
>
>		Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume of 960 ml. Autoclave.
 

Revision 12012-01-11 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

SOB: Super Optimal Broth

1L Final [ ] Component
5 g 0.5% yeast extract
20 g 2.0% tryptone
0.5 g 10 mM NaCl
0.186 g 2 mM KCl
2.4 g 20 mM MgSO4

Add 800 ml of water and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add H2O to a final volume of 960 ml. Autoclave.

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