(r1) Barrick Lab :: ProtocolsRecipesSOB

---+++ SOB: Super Optimal Broth



%TABLE{}%
|  *1L*  |  *Final [ ]*  |  *Component*  |
|  5 g   | 0.5% | yeast extract | 
|  20 g   | 2.0% | tryptone | 
| 0.5 g | 10 mM | NaCl |
| 0.186 g | 2 mM | KCl |
| 2.4 g | 20 mM | MgSO<sub>4</sub> |

Add 800 ml of water and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add H<sub>2</sub>O to a final volume of 960 ml. Autoclave.