(r6) ProtocolsRecipesS2 < Lab

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---+++ S2

Used for Acinetobacter

|  *1L*  |  *5L*  |  *Component*  |
| 1.5g | 7.5 g | KH<sub>2</sub>PO<sub>4</sub>| 
| 25.5g | 127.5 g | Na<sub>2</sub>HPO<sub>4</sub> 7H<sub>2</sub>O| 
| 2g | 10 g | NH<sub>4</sub>Cl| 

After autoclaving, wait for containers to return to room temperature, then add the following sterile ingredients.

|  *1L*  |  *Component*  |
| 1ml | 10% MgSO<sub>4</sub> | 
| 0.09ul | 1M CaCl<sub>2</sub> | 
| 0.5ml | 0.1% FeSO<sub>4</sub> 7H<sub>2</sub>O |
| 5 ml | 90% Lactic Acid | 

The functional constraints are that Calcium Chloride cannot be autoclaved in the presence of sulfates, or it will precipitate as calcium sulfate. Ferrous sulfate when autoclaved will decompose to ferric sulfate, ferric oxide and sulfur dioxide. This means that either calcium chloride OR magnesium sulfate may be added before autoclaving, but not both.

| *Final composition* ||
| 190.2 mM | Sodium (Na<sup>+</sup>) |
| 11.0 mM | Potassium (K<sup>+</sup>) |
| 37.8 mM | Ammonium (NH<sub>4</sub><sup>+</sup>) | 
| 0.09 mM | Calcium (Ca<sup>2+</sup>) |
| 0.83 mM | Magnesium (Mg<sup>2+</sup>) |
| 0.033 mM | Iron II (Fe<sup>2+</sup>) |
| 38.0 mM | Chloride (Cl<sup>-</sup>)|
| 0.87 mM | Sulfate (SO<sub>4</sub><sup>2-</sup>) |
| 106.1 mM | Phosphate (PO<sub>4</sub><sup>3-</sup>) |
| 50.0 mM | Lactic Acid|

-- Main.AurkoDasgupta - 03 Jun 2013
Barrick Lab > ProtocolsRecipesS2
Topic revision: r6 - 2013-06-27 - 15:18:45 - Main.AurkoDasgupta