S2

Used for Acinetobacter

1L 5L Component
1.5g 7.5 g KH2PO4
25.5g 127.5 g Na2HPO4 7H2O
0.1g 0.5 g MgSO4
2g 10 g NH4Cl
0.01g 0.05 g CaCl2
0.0005g 0.0025 g FeSO4 7H2O

To each 1L, add 5ml of 85-90% lactic acid. Bring final pH to 6.65.

Calcium Chloride may be better added as 1ml of 1% solution, or 90ul of 1M solution.

Ferrous Sulfate may be better added as 0.5ml of a 0.1% solution.

Final composition
95.1 mM Sodium (Na+)
11.0 mM Potassium (K+)
37.8 mM Ammonium (NH4)
0.09 mM Calcium (Ca2+)
0.83 mM Magnesium (Mg2+)
38.0 mM Chloride (Cl-
0.87 mM Sulfate (SO42-)
106.1 mM Phosphate (PO43-)
50.0 mM Lactic Acid

-- Main.AurkoDasgupta - 03 Jun 2013

Edit | Attach | Watch | Print version | History: r7 | r5 < r4 < r3 < r2 | Backlinks | Raw View | More topic actions...

 Barrick Lab  >  ProtocolsRecipesS2

Contributors to this topic Edit topic AurkoDasgupta, GabrielSuarez
Topic revision: r3 - 2013-06-21 - 20:59:47 - Main.AurkoDasgupta
 
This site is powered by the TWiki collaboration platform Powered by Perl This site is powered by the TWiki collaboration platformCopyright ©2025 Barrick Lab contributing authors. Ideas, requests, problems? Send feedback