(r2) Barrick Lab :: ProtocolsRecipesLysogenyBrothAgar

---+++ LB: Luria-Broth

Combine in a 2 L flask:
| 15 g | Tryptone | 
| 7.5 g | Yeast Extract |
| 15 g | NaCl | 
| 24 g | Agar | 
| 1.5 ml | 5% Antifoam | 

Add dH<sub>2</sub>O to 1.5 L. Autoclave.

*Note: This is _different_ from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!*

To make "soft" agar for phage studies, use only 7 g agar per liter.

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Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesLysogenyBrothAgar

Contributors to this topic

JeffreyBarrick, CraigBarnhart, TylerDeJong, SophiaLin, AlvaroRodriguez

Topic revision: r2 - 2011-07-26 - 12:12:25 - Main.CraigBarnhart

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