LB: Luria-Broth

Combine in a 2 L flask the following:

1.5L Component
15 g Tryptone
7.5 g Yeast Extract
15 g NaCl
24 g Agar
1.5 ml 5% Antifoam

Add dH2O to 1.5 L. Autoclave.

Note: This is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!

To make "soft" agar for phage studies, use only 7 g agar per liter.

Edit | Attach | Watch | Print version | History: r8 | r5 < r4 < r3 < r2 | Backlinks | Raw View | More topic actions...

 Barrick Lab  >  ProtocolsMediaRecipes  >  ProtocolsRecipesLysogenyBrothAgar

Contributors to this topic Edit topic JeffreyBarrick, CraigBarnhart, AlvaroRodriguez
Topic revision: r3 - 2011-07-29 - 14:42:42 - Main.CraigBarnhart
 
This site is powered by the TWiki collaboration platform Powered by Perl This site is powered by the TWiki collaboration platformCopyright ©2024 Barrick Lab contributing authors. Ideas, requests, problems? Send feedback