LB: Luria-Broth

Combine in a 2 L flask:

15 g Tryptone
7.5 g Yeast Extract
15 g NaCl
24 g Agar
1.5 ml 5% Antifoam or .75 ml 10% Antifoam

Add dH2O to 1.5 L. Autoclave.

Note: This is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!

To make "soft" agar for phage studies, use only 7 g agar per liter.

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 Barrick Lab  >  ProtocolsMediaRecipes  >  ProtocolsRecipesLysogenyBrothAgar

Contributors to this topic Edit topic JeffreyBarrick, CraigBarnhart, TylerDeJong, AlvaroRodriguez, SophiaLin
Topic revision: r1 - 2011-06-14 - 12:40:09 - Main.CraigBarnhart
 
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