---+++ LB: Luria-Broth Combine in a 2 L flask: | 15 g | Tryptone | | 7.5 g | Yeast Extract | | 15 g | NaCl | | 24 g | Agar | | 1.5 ml | 5% Antifoam | or | .75 ml | 10% Antifoam | Add dH<sub>2</sub>O to 1.5 L. Autoclave. *Note: This is _different_ from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!* To make "soft" agar for phage studies, use only 7 g agar per liter.
Edit
|
Attach
|
P
rint version
V
iew topic
|
Backlinks:
We
b
,
A
l
l Webs
|
H
istory
:
r11
|
r4
<
r3
<
r2
<
r1
|
Refresh
|
More topic actions...
Barrick Lab
>
ProtocolsMediaRecipes
>
ProtocolsRecipesLysogenyBrothAgar
Topic revision: r1 - 2011-06-14 - 12:40:09 - Main.CraigBarnhart
Barrick Lab
Homepage
Contact Information
Michigan State University
Department of Microbiology, Genetics, & Immunology
Department of Entomology
Ecology, Evolution, and Behavior Graduate Program
UT Austin iGEM team
Team
Research
Overview
Publications
Software
Protocols
Protocol List
Reference Information
The LTEE
Copyright ©2025 Barrick Lab contributing authors. Ideas, requests, problems?
Send feedback