LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

Combine in a 2 L flask the following:

Add dH₂O to 1.5 L. Autoclave.

Notes and variations:

• Common synonyms are Luria-Broth and Luria-Bertani medium.
• This is the "Miller" formulation of LB with 10 g NaCl per liter (1% w/v). Another common formulation has only 5 g NaCl per liter (0.5% w/v)! They are NOT interchangeable.
• If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
• To make "soft" or "top" agar for phage studies, use only 7-8 g agar per liter.