LB: Lysogeny Broth / Luria-Bertani (Miller) Agar
Combine in a 2 L flask the following:
1.5L |
Component |
15 g |
Tryptone |
7.5 g |
Yeast Extract |
15 g |
NaCl |
24 g |
Agar |
1.5 ml |
5% Antifoam |
Add dH
2O to 1.5 L. Autoclave.
Notes and variations:
- Common synonyms are Luria-Broth and Luria-Bertani medium.
- This is the "Miller" formulation of LB with 10 g NaCl per liter (1% w/v). Another common formulation has only 5 g NaCl per liter (0.5% w/v)! They are NOT interchangeable.
- If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
- To make "soft" or "top" agar for phage studies, use only 7-8 g agar per liter.
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Topic revision: r8 - 2020-02-11 - JeffreyBarrick