(r5) ProtocolsRecipesDavisMingioli < Lab

---+++ DM: Davis Minimal

|  *1L*  |  *5L*  |  *Component*  |  *MW*  |
|  7 g  |  35 g  | Potassium Phosphate (dibasic, trihydrate) HK<sub>2</sub>PO<sub>4</sub> (H<sub>2</sub>O)<sub>3</sub> |  MW 228.17 |  
|  2 g  | 10 g | Potassium Phosphate (monobasic) H<sub>2</sub>KPO<sub>4</sub> | MW 136.07 |
|  1 g  | 5 g | Ammonium Sulfate (NH<sub>4</sub>)<sub>2</sub> SO<sub>4</sub>| MW 132.08 |
|  0.5 g  | 2.5 g | Sodium Citrate (trisodium, dihydrate) Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>  (H<sub>2</sub>O)<sub>2</sub>| MW 294.10 |
Add dH<sub>2</sub>O to final volume and autoclave. <br>

After autoclaving add the following stock solutions: <br>

|  *1L*  |  *5L*  |  *Component*  |  *MW*  |
| 1.0 ml | 5 ml | 10% Magnesium Sulfate MgSO<sub>4</sub> |  MW 120.37  | | 
| 1.0 ml | 5 ml | 0.2% Thiamin (vitamin B1) | Note* Thiamin must be syringe filtered into the media | |

Final nutrient concentrations:
| 67.4 mM | Phosphate (PO<sub>4</sub><sup>3-</sup>) |
| 8.38 mM | Sulfate (SO<sub>4</sub><sup>2-</sup>) |
| 15.1 mM | Ammonium (NH<sub>4</sub>) | 
| 5.1 mM | Sodium (Na<sup>+</sup>) |
| 98.1 mM | Potassium (K<sup>+</sup>) |
| 0.83 mM | Magnesium (MGgsup>2+</sup>) |
| 1.7 mM | Citrate |
| 139 µM | Glucose (in DM25) |

Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesDavisMingioli

Topic revision: r5 - 2011-07-29 - 14:13:03 - Main.CraigBarnhart