DM: Davis Minimal
| 1L | 5L | Component | MW |
|---|---|---|---|
| 7 g | 35 g | Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 | MW 228.17 |
| 2 g | 10 g | Potassium Phosphate (monobasic) H2KPO4 | MW 136.07 |
| 1 g | 5 g | Ammonium Sulfate (NH4)2 SO4 | MW 132.08 |
| 0.5 g | 2.5 g | Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 | MW 294.10 |
After autoclaving add the following stock solutions:
| 1L | 5L | Component |
|---|---|---|
| 1.0 ml | 5 ml | 10% Magnesium Sulfate MgSO4 |
| 1.0 ml | 5 ml | 0.2% Thiamin (vitamin B1) (filter sterilized) |
| 67.4 mM | Phosphate (PO43-) |
| 8.38 mM | Sulfate (SO42-) |
| 15.1 mM | Ammonium (NH4) |
| 5.1 mM | Sodium (Na+) |
| 98.1 mM | Potassium (K+) |
| 0.83 mM | Magnesium (MG2+) |
| 1.7 mM | Citrate |
| 139 µM | Glucose (in DM25) |
Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesDavisMingioli
Topic revision: r6 - 2011-10-05 - 21:58:16 - Main.JeffreyBarrick
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