Difference: ProtocolsRecipesDavisMingioli (1 vs. 23)

Revision 232025-05-20 - AlexaMorton

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

Changed:
<
<
0.5L 1L 4.4L 5L Component MW
2.67 g 5.34 g 23.32 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
1 g 2 g 8 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
0.5 g 1 g 4.4 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.25 g 0.5 g 2.2 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
>
>
0.5L 1L 4.5L 5L Component MW
2.67 g 5.34 g 24.03 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
1 g 2 g 9 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
0.5 g 1 g 4.5 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.25 g 0.5 g 2.25 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
 Add dH2O to final volume and autoclave.
Changed:
<
<		Note: 4.4 L of DM makes 8 bottles with 550 ml each.
>
>		Note: 4.5 L of DM makes 8 bottles with 550 ml each.
 
Changed:
<
<		Note: If using Potassium phosphate (dibasic) trihydrate (MW 228.22) use 7g per L
>
>		Note: If using Potassium phosphate (dibasic) trihydrate (MW 228.22) use 7g per L
  After autoclaving add the following stock solutions:

0.5L 550mL 1L 5L Component
0.5 ml 550 痞 1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
Changed:
<
<
0.5 ml 1000 痞 1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)
>
>
0.5 ml 550 痞 1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)
  And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

0.5L 550mL 1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
125 痞 137.5 痞 250 痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
0.5 ml 0.55 ml 1 ml 5 ml DM100 0.010% 100 mg/L 694 然
1.25 ml 1.375 ml 2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
2.5 ml 2.75 ml 5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
5 ml 5.5 ml 10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
10 ml 11 ml 20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM

Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition
5.1 mM Sodium (Na+)
75.8 mM Potassium (K+)
15.2 mM Ammonium (NH4)
0.83 mM Magnesium (Mg2+)
8.41 mM Sulfate (SO42-)
45.3 mM Phosphate (PO43-)
1.70 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 222025-05-20 - AlexaMorton

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

0.5L 1L 4.4L 5L Component MW
2.67 g 5.34 g 23.32 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
1 g 2 g 8 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
0.5 g 1 g 4.4 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.25 g 0.5 g 2.2 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

Note: 4.4 L of DM makes 8 bottles with 550 ml each.

Note: If using Potassium phosphate (dibasic) trihydrate (MW 228.22) use 7g per L

Deleted:
<
<
 After autoclaving add the following stock solutions:

0.5L 550mL 1L 5L Component
Changed:
<
<
0.5 ml 550 痞 1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
>
>
0.5 ml 550 痞 1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
 
0.5 ml 1000 痞 1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)

And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

0.5L 550mL 1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
125 痞 137.5 痞 250 痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
Changed:
<
<
0.5 ml 0.55 ml 1 ml 5 ml DM100 0.010% 100 mg/L 694 然
>
>
0.5 ml 0.55 ml 1 ml 5 ml DM100 0.010% 100 mg/L 694 然
 
1.25 ml 1.375 ml 2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
2.5 ml 2.75 ml 5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
5 ml 5.5 ml 10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
10 ml 11 ml 20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM

Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition
5.1 mM Sodium (Na+)
75.8 mM Potassium (K+)
Changed:
<
<
15.2 mM Ammonium (NH4)
>
>
15.2 mM Ammonium (NH4)
 
0.83 mM Magnesium (Mg2+)
8.41 mM Sulfate (SO42-)
45.3 mM Phosphate (PO43-)
1.70 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 212024-09-10 - SophiaLin

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

Changed:
<
<
0.5L 1L 5L Component MW
2.67 g 5.34 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
1 g 2 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
0.5 g 1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.25 g 0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
>
>
0.5L 1L 4.4L 5L Component MW
2.67 g 5.34 g 23.32 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
1 g 2 g 8 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
0.5 g 1 g 4.4 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.25 g 0.5 g 2.2 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
 Add dH2O to final volume and autoclave.
Changed:
<
<		Note: if using Potassium phosphate (dibasic) trihydrate (MW 228.22) use 7g per L
>
>		Note: 4.4 L of DM makes 8 bottles with 550 ml each.
 
Added:
>
>		Note: If using Potassium phosphate (dibasic) trihydrate (MW 228.22) use 7g per L
 
Deleted:
<
<		After autoclaving add the following stock solutions:
 
Changed:
<
<
0.5L 1L 5L Component
>
>		After autoclaving add the following stock solutions:
Deleted:
<
<
0.5 ml 1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
0.5 ml 1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)
 
Added:
>
>
0.5L 550mL 1L 5L Component
0.5 ml 550 痞 1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
0.5 ml 1000 痞 1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)
 And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

Changed:
<
<
0.5L 1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
125 痞 250 痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
0.5 ml 1 ml 5 ml DM100 0.010% 100 mg/L 694 然
1.25 ml 2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
2.5 ml 5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
5 ml 10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
10 ml 20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM
>
>
0.5L 550mL 1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
125 痞 137.5 痞 250 痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
0.5 ml 0.55 ml 1 ml 5 ml DM100 0.010% 100 mg/L 694 然
1.25 ml 1.375 ml 2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
2.5 ml 2.75 ml 5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
5 ml 5.5 ml 10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
10 ml 11 ml 20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM
  Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition
5.1 mM Sodium (Na+)
75.8 mM Potassium (K+)
15.2 mM Ammonium (NH4)
0.83 mM Magnesium (Mg2+)
8.41 mM Sulfate (SO42-)
45.3 mM Phosphate (PO43-)
1.70 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 202022-09-21 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

Changed:
<
<
1L 5L Component MW
5.3 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
>
>
0.5L 1L 5L Component MW
2.67 g 5.34 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
1 g 2 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
0.5 g 1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.25 g 0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
 Add dH2O to final volume and autoclave.
Changed:
<
<		Note: if using Potassium phosphate (dibasic) trihydrate use 7g per L
>
>		Note: if using Potassium phosphate (dibasic) trihydrate (MW 228.22) use 7g per L
 

After autoclaving add the following stock solutions:

Changed:
<
<
1L 5L Component
1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)
>
>
0.5L 1L 5L Component
0.5 ml 1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
0.5 ml 1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)
  And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

Changed:
<
<
1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
250痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
1 ml 5 ml DM100 0.010% 100 mg/L 694 然
2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM
>
>
0.5L 1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
125 痞 250 痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
0.5 ml 1 ml 5 ml DM100 0.010% 100 mg/L 694 然
1.25 ml 2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
2.5 ml 5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
5 ml 10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
10 ml 20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM
  Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition
5.1 mM Sodium (Na+)
75.8 mM Potassium (K+)
15.2 mM Ammonium (NH4)
0.83 mM Magnesium (Mg2+)
8.41 mM Sulfate (SO42-)
45.3 mM Phosphate (PO43-)
1.70 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 192015-06-26 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

1L 5L Component MW
5.3 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

Note: if using Potassium phosphate (dibasic) trihydrate use 7g per L

After autoclaving add the following stock solutions:

1L 5L Component
1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)

And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
250痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
1 ml 5 ml DM100 0.010% 100 mg/L 694 然
2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM

Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition
5.1 mM Sodium (Na+)
75.8 mM Potassium (K+)
15.2 mM Ammonium (NH4)
0.83 mM Magnesium (Mg2+)
8.41 mM Sulfate (SO42-)
45.3 mM Phosphate (PO43-)
Changed:
<
<
1.7 mM Citrate
>
>
1.70 mM Citrate
 
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 182014-06-28 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

1L 5L Component MW
5.3 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

Note: if using Potassium phosphate (dibasic) trihydrate use 7g per L

After autoclaving add the following stock solutions:

1L 5L Component
1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)

And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
250痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
1 ml 5 ml DM100 0.010% 100 mg/L 694 然
2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM

Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Changed:
<
<
Final composition
>
>
Final composition
Deleted:
<
<
 
5.1 mM Sodium (Na+)
75.8 mM Potassium (K+)
15.2 mM Ammonium (NH4)
0.83 mM Magnesium (Mg2+)
8.41 mM Sulfate (SO42-)
45.3 mM Phosphate (PO43-)
1.7 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 172013-08-12 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

1L 5L Component MW
5.3 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.
Added:
>
>		 Note: if using Potassium phosphate (dibasic) trihydrate use 7g per L
  After autoclaving add the following stock solutions:

1L 5L Component
1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)

And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
250痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
1 ml 5 ml DM100 0.010% 100 mg/L 694 然
2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM

Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition

5.1 mM Sodium (Na+)
75.8 mM Potassium (K+)
15.2 mM Ammonium (NH4)
0.83 mM Magnesium (Mg2+)
8.41 mM Sulfate (SO42-)
45.3 mM Phosphate (PO43-)
1.7 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 162013-06-25 - AurkoDasgupta

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

1L 5L Component MW
5.3 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

After autoclaving add the following stock solutions:

1L 5L Component
1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)

And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
250痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
1 ml 5 ml DM100 0.010% 100 mg/L 694 然
2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM

Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition

5.1 mM Sodium (Na+)
75.8 mM Potassium (K+)
15.2 mM Ammonium (NH4)
0.83 mM Magnesium (Mg2+)
8.41 mM Sulfate (SO42-)
Changed:
<
<
45.1 mM Phosphate (PO43-)
>
>
45.3 mM Phosphate (PO43-)
 
1.7 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 152013-06-25 - AurkoDasgupta

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

1L 5L Component MW
5.3 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

After autoclaving add the following stock solutions:

1L 5L Component
1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)

And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
250痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
1 ml 5 ml DM100 0.010% 100 mg/L 694 然
2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM

Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition

5.1 mM Sodium (Na+)
Changed:
<
<
98.1 mM Potassium (K+)
>
>
75.8 mM Potassium (K+)
 
15.2 mM Ammonium (NH4)
0.83 mM Magnesium (Mg2+)
8.41 mM Sulfate (SO42-)
Changed:
<
<
67.4 mM Phosphate (PO43-)
>
>
45.1 mM Phosphate (PO43-)
 
1.7 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 142013-06-24 - AurkoDasgupta

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

1L 5L Component MW
5.3 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

After autoclaving add the following stock solutions:

1L 5L Component
1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)

And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
250痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
1 ml 5 ml DM100 0.010% 100 mg/L 694 然
2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM

Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition

5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
Changed:
<
<
15.1 mM Ammonium (NH4)
>
>
15.2 mM Ammonium (NH4)
 
0.83 mM Magnesium (Mg2+)
Changed:
<
<
8.38 mM Sulfate (SO42-)
>
>
8.41 mM Sulfate (SO42-)
 
67.4 mM Phosphate (PO43-)
1.7 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 132013-06-21 - AurkoDasgupta

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

1L 5L Component MW
5.3 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

After autoclaving add the following stock solutions:

1L 5L Component
1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)

And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
250痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
1 ml 5 ml DM100 0.010% 100 mg/L 694 然
2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM

Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition
Changed:
<
<
67.4 mM Phosphate (PO43-)
>
>
Deleted:
<
<
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
 
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
Added:
>
>
15.1 mM Ammonium (NH4)
 
0.83 mM Magnesium (Mg2+)
Added:
>
>
8.38 mM Sulfate (SO42-)
67.4 mM Phosphate (PO43-)
 
1.7 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 122013-02-19 - AurkoDasgupta

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

1L 5L Component MW
Changed:
<
<
7 g 35 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 10 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
>
>
5.3 g 26.7 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 10 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
 
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

After autoclaving add the following stock solutions:

1L 5L Component
1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)

And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
250痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
1 ml 5 ml DM100 0.010% 100 mg/L 694 然
2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM

Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition
67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
0.83 mM Magnesium (Mg2+)
1.7 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 112012-11-20 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

1L 5L Component MW
7 g 35 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 10 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

After autoclaving add the following stock solutions:

1L 5L Component
1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)

And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
Changed:
<
<
25 痞 125 痞 DM25 0.0025% 25 mg/L 139 然
100 痞 500 痞 DM100 0.010% 100 mg/L 694 然
250 痞 1.25 ml DM250 0.025% 250 mg/L 1.39 然
500 痞 2.5 ml DM500 0.05% 500 mg/L 2.78 mM
1.0 ml 5 ml DM1000 0.1% 1000 mg/L 5.55 mM
2.0 ml 10 ml DM2000 0.2% 2000 mg/L 11.1 mM
>
>
250痞 1.25 ml DM25 0.0025% 25 mg/L 139 然
1 ml 5 ml DM100 0.010% 100 mg/L 694 然
2.5 ml 12.5 ml DM250 0.025% 250 mg/L 1.39 然
5 ml 25 ml DM500 0.05% 500 mg/L 2.78 mM
10 ml 50 ml DM1000 0.1% 1000 mg/L 5.55 mM
20 ml 100 ml DM2000 0.2% 2000 mg/L 11.1 mM
  Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition
67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
0.83 mM Magnesium (Mg2+)
1.7 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 102012-10-29 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis-Mingioli

Growth medium used by the long-term E. coli evolution experiment.

1L 5L Component MW
7 g 35 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 10 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

After autoclaving add the following stock solutions:

1L 5L Component
1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)

And supplement with a carbon source.

If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

Changed:
<
<
1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (然)
25 痞 125 痞 DM25 0.025% 25 mg/L 139 然
100 痞 500 痞 DM100 0.100% 100 mg/L 694 然
250 痞 1.25 ml DM250 0.250% 250 mg/L 1.39 然
500 痞 2.5 ml DM500 0.500% 500 mg/L 2.78 mM
1.0 ml 5 ml DM1000 1.000% 1000 mg/L 5.55 mM
2.0 ml 10 ml DM2000 2.000% 2000 mg/L 11.1 mM
>
>
1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (M)
25 痞 125 痞 DM25 0.0025% 25 mg/L 139 然
100 痞 500 痞 DM100 0.010% 100 mg/L 694 然
250 痞 1.25 ml DM250 0.025% 250 mg/L 1.39 然
500 痞 2.5 ml DM500 0.05% 500 mg/L 2.78 mM
1.0 ml 5 ml DM1000 0.1% 1000 mg/L 5.55 mM
2.0 ml 10 ml DM2000 0.2% 2000 mg/L 11.1 mM
  Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition
67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
0.83 mM Magnesium (Mg2+)
1.7 mM Citrate
139 然 Glucose (in DM25)

META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"

Revision 92012-10-28 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"
Changed:
<
<

DM: Davis Minimal

>
>

DM: Davis-Mingioli

 
Added:
>
>		Growth medium used by the long-term E. coli evolution experiment.
 
1L 5L Component MW
7 g 35 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 10 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

After autoclaving add the following stock solutions:

1L 5L Component
Changed:
<
<
1.0 ml 5 ml 10% Magnesium Sulfate MgSO4
1.0 ml 5 ml 0.2% Thiamine (vitamin B1) (filter sterilized)
>
>
1.0 ml 5 ml 10% (w/v) Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 5 ml 0.2% (w/v) Thiamine (vitamin B1) (filter sterilized)
 
Changed:
<
<		Final nutrient concentrations:
>
>		And supplement with a carbon source.
Added:
>
>		 If preparing DM-glucose (MW 180.16 g/mol), add this volume of 10% glucose solution (separately autoclaved stock) to get the final concentration desired:

1L 5L DMX [glucose] (w/v) [glucose] (mg/L) [glucose] (然)
25 痞 125 痞 DM25 0.025% 25 mg/L 139 然
100 痞 500 痞 DM100 0.100% 100 mg/L 694 然
250 痞 1.25 ml DM250 0.250% 250 mg/L 1.39 然
500 痞 2.5 ml DM500 0.500% 500 mg/L 2.78 mM
1.0 ml 5 ml DM1000 1.000% 1000 mg/L 5.55 mM
2.0 ml 10 ml DM2000 2.000% 2000 mg/L 11.1 mM

Remember: DMX = DM + X mg/L glucose. Glucose may no longer limit the final growth density above approximately DM1000.

Final composition
 
67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
0.83 mM Magnesium (Mg2+)
1.7 mM Citrate
139 然 Glucose (in DM25)
Added:
>
>
META TOPICMOVED by="JeffreyBarrick" date="1351456485" from="Lab.ProtocolsRecipesDavisMinimal" to="Lab.ProtocolsRecipesDavisMingioli"
 

Revision 82012-10-01 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis Minimal

1L 5L Component MW
7 g 35 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 10 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

After autoclaving add the following stock solutions:

1L 5L Component
1.0 ml 5 ml 10% Magnesium Sulfate MgSO4
1.0 ml 5 ml 0.2% Thiamine (vitamin B1) (filter sterilized)

Final nutrient concentrations:

67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
Changed:
<
<
0.83 mM Magnesium (MG2+)
>
>
0.83 mM Magnesium (Mg2+)
 
1.7 mM Citrate
139 然 Glucose (in DM25)

Revision 72012-05-02 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis Minimal

1L 5L Component MW
7 g 35 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 10 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

After autoclaving add the following stock solutions:

1L 5L Component
1.0 ml 5 ml 10% Magnesium Sulfate MgSO4
Changed:
<
<
1.0 ml 5 ml 0.2% Thiamin (vitamin B1) (filter sterilized)
>
>
1.0 ml 5 ml 0.2% Thiamine (vitamin B1) (filter sterilized)
  Final nutrient concentrations:
67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
0.83 mM Magnesium (MG2+)
1.7 mM Citrate
139 然 Glucose (in DM25)

Revision 62011-10-05 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis Minimal

1L 5L Component MW
7 g 35 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 10 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.

After autoclaving add the following stock solutions:

Changed:
<
<
1L 5L Component MW
1.0 ml 5 ml 10% Magnesium Sulfate MgSO4 MW 120.37  
1.0 ml 5 ml 0.2% Thiamin (vitamin B1) Note* Thiamin must be syringe filtered into the media  
>
>
1L 5L Component
1.0 ml 5 ml 10% Magnesium Sulfate MgSO4
1.0 ml 5 ml 0.2% Thiamin (vitamin B1) (filter sterilized)
  Final nutrient concentrations:
67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
Changed:
<
<
0.83 mM Magnesium (MGgsup>2+)
>
>
0.83 mM Magnesium (MG2+)
 
1.7 mM Citrate
139 然 Glucose (in DM25)

Revision 52011-07-29 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis Minimal

Changed:
<
<		 Notes: Salts, agar and sugars need to be prepared and autoclaved separately.
>
>
1L 5L Component MW
Added:
>
>
7 g 35 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 10 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 2.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to final volume and autoclave.
 
Changed:
<
<		For making 1.5 liters of DM agar do the following:
>
>		After autoclaving add the following stock solutions:
 
Changed:
<
<		Combine the following in a 2 L flask (including magnetic stir bar):

10.5 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
>
>
1L 5L Component MW
1.0 ml 5 ml 10% Magnesium Sulfate MgSO4 MW 120.37  
1.0 ml 5 ml 0.2% Thiamin (vitamin B1) Note* Thiamin must be syringe filtered into the media  
Deleted:
<
<
3 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add 500 ml of dH2O, cover with tin foil and autoclave.

Next, prepare the agar in a 1 liter flask (including magnetic stir bar) by adding the following:

24 g Agar
1.5 ml Antifoam (5%)

Add 500 ml of dH2O, cover with tin foil and autoclave.

Next, prepare the sugar in a separate 1 liter flask by adding the following:

6 g Glucose (or other sugar)

Add 500 ml of dH2O, cover with tin foil and autoclave.

After autoclaving combine the contents of the three flasks into the larger flask and add the following stock solutions:

1.5 ml 10% Magnesium Sulfate MgSO4 MW 120.37  
1.5 ml 0.2% Thiamin (vitamin B1) Note* Thiamin must be syringe filtered into the media  

To make 1 liter of DM plates combine the following in a 2 L flask (including magnetic stir bar):

7 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add about 333 ml of dH2O, cover with tin foil and autoclave.

Next, prepare the agar in a 1 liter flask (including magnetic stir bar) by adding the following:

16 g Agar
1 ml Antifoam (5%)

Add about 333 ml of dH2O, cover with tin foil and autoclave.

Next, prepare the sugar in a separate 1 liter flask by adding the following:

4 g Glucose (or other sugar)

Add about 333 ml of dH2O, cover with tin foil and autoclave.

After autoclaving combine the contents of the three flasks into the larger flask and add the following stock solutions:

1 ml 10% Magnesium Sulfate MgSO4 MW 120.37  
1 ml 0.2% Thiamin (vitamin B1) Note* Thiamin must be syringe filtered into the media  
  Final nutrient concentrations:
67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
0.83 mM Magnesium (MGgsup>2+)
1.7 mM Citrate
139 然 Glucose (in DM25)

Revision 42011-07-26 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis Minimal

Changed:
<
<		Notes: Salts, agar and sugars need to be prepared and autoclaved separately.
>
>		 Notes: Salts, agar and sugars need to be prepared and autoclaved separately.
 
Changed:
<
<		For making 1.5 liters of DM agar do the following:
>
>		For making 1.5 liters of DM agar do the following:
  Combine the following in a 2 L flask (including magnetic stir bar):

10.5 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
3 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add 500 ml of dH2O, cover with tin foil and autoclave.

Next, prepare the agar in a 1 liter flask (including magnetic stir bar) by adding the following:

24 g Agar
1.5 ml Antifoam (5%)

Add 500 ml of dH2O, cover with tin foil and autoclave.

Next, prepare the sugar in a separate 1 liter flask by adding the following:

6 g Glucose (or other sugar)

Add 500 ml of dH2O, cover with tin foil and autoclave.

After autoclaving combine the contents of the three flasks into the larger flask and add the following stock solutions:

1.5 ml 10% Magnesium Sulfate MgSO4 MW 120.37  
1.5 ml 0.2% Thiamin (vitamin B1) Note* Thiamin must be syringe filtered into the media  
Changed:
<
<		To make 1 liter of DM plates combine the following in a 2 L flask (including magnetic stir bar):
>
>		 To make 1 liter of DM plates combine the following in a 2 L flask (including magnetic stir bar):
 
7 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add about 333 ml of dH2O, cover with tin foil and autoclave.

Next, prepare the agar in a 1 liter flask (including magnetic stir bar) by adding the following:

16 g Agar
1 ml Antifoam (5%)

Add about 333 ml of dH2O, cover with tin foil and autoclave.

Next, prepare the sugar in a separate 1 liter flask by adding the following:

4 g Glucose (or other sugar)

Add about 333 ml of dH2O, cover with tin foil and autoclave.

After autoclaving combine the contents of the three flasks into the larger flask and add the following stock solutions:

1 ml 10% Magnesium Sulfate MgSO4 MW 120.37  
1 ml 0.2% Thiamin (vitamin B1) Note* Thiamin must be syringe filtered into the media  

Final nutrient concentrations:

67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
0.83 mM Magnesium (MGgsup>2+)
1.7 mM Citrate
139 然 Glucose (in DM25)

Revision 32011-07-26 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis Minimal

Added:
>
>		Notes: Salts, agar and sugars need to be prepared and autoclaved separately.

For making 1.5 liters of DM agar do the following:

Combine the following in a 2 L flask (including magnetic stir bar):

10.5 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
3 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add 500 ml of dH2O, cover with tin foil and autoclave.

Next, prepare the agar in a 1 liter flask (including magnetic stir bar) by adding the following:

24 g Agar
1.5 ml Antifoam (5%)

Add 500 ml of dH2O, cover with tin foil and autoclave.

Next, prepare the sugar in a separate 1 liter flask by adding the following:

6 g Glucose (or other sugar)

Add 500 ml of dH2O, cover with tin foil and autoclave.

After autoclaving combine the contents of the three flasks into the larger flask and add the following stock solutions:

1.5 ml 10% Magnesium Sulfate MgSO4 MW 120.37  
1.5 ml 0.2% Thiamin (vitamin B1) Note* Thiamin must be syringe filtered into the media  

To make 1 liter of DM plates combine the following in a 2 L flask (including magnetic stir bar):

 
7 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Changed:
<
<		Add dH2O to 1 liter. Autoclave. Then add
1 ml 10% Magnesium Sulfate MgSO4 MW 120.37 Note* The MgSO4 must be autoclaved separately.
1 ml 0.2% Thiamin (vitamin B1)  
>
>		Add about 333 ml of dH2O, cover with tin foil and autoclave.

Next, prepare the agar in a 1 liter flask (including magnetic stir bar) by adding the following:

Added:
>
>
16 g Agar
1 ml Antifoam (5%)

Add about 333 ml of dH2O, cover with tin foil and autoclave.

Next, prepare the sugar in a separate 1 liter flask by adding the following:

4 g Glucose (or other sugar)

Add about 333 ml of dH2O, cover with tin foil and autoclave.

After autoclaving combine the contents of the three flasks into the larger flask and add the following stock solutions:

1 ml 10% Magnesium Sulfate MgSO4 MW 120.37  
1 ml 0.2% Thiamin (vitamin B1) Note* Thiamin must be syringe filtered into the media  
  Final nutrient concentrations:
67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
0.83 mM Magnesium (MGgsup>2+)
1.7 mM Citrate
139 然 Glucose (in DM25)

Revision 22011-07-19 - BrianRenda

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis Minimal

7 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
Changed:
<
<
5 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
>
>
2 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
 
1 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to 1 liter. Autoclave. Then add
1 ml 10% Magnesium Sulfate MgSO4 MW 120.37 Note* The MgSO4 must be autoclaved separately.
1 ml 0.2% Thiamin (vitamin B1)  

Final nutrient concentrations:

67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
0.83 mM Magnesium (MGgsup>2+)
1.7 mM Citrate
139 然 Glucose (in DM25)

Revision 12011-06-14 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

DM: Davis Minimal

7 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
5 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to 1 liter. Autoclave. Then add
1 ml 10% Magnesium Sulfate MgSO4 MW 120.37 Note* The MgSO4 must be autoclaved separately.
1 ml 0.2% Thiamin (vitamin B1)  

Final nutrient concentrations:

67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
0.83 mM Magnesium (MGgsup>2+)
1.7 mM Citrate
139 然 Glucose (in DM25)
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