breseq version 0.35.7 revision b7a44cd89118
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 34 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 2,584,958 | 0 | T | G | 61.5% | ‑2.1 / 12.7 | 13 | intergenic (+14/‑104) | suhB/yfhR | inositol monophosphatase/predicted peptidase |
* | REL606 | 4,549,418 | 0 | T | G | 34.7% | 146.5 / 14.3 | 72 | T6P (ACC→CCC) | yjiO | multidrug efflux system protein |
* | REL606 | 4,335,161 | 0 | T | G | 34.2% | 41.3 / 11.3 | 38 | intergenic (‑30/+29) | yjdL/cadA | predicted transporter/lysine decarboxylase 1 |
* | REL606 | 1,292,259 | 0 | T | C | 32.8% | 114.0 / 23.7 | 67 | intergenic (+100/+44) | galU/hns | glucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS |
* | REL606 | 1,819,959 | 0 | A | C | 32.7% | 113.8 / 10.0 | 52 | T121P (ACC→CCC) | gdhA | glutamate dehydrogenase |
* | REL606 | 280 | 0 | G | C | 32.0% | 98.7 / 11.7 | 50 | intergenic (+25/‑56) | thrL/thrA | thr operon leader peptide/bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 2,526,164 | 0 | G | C | 31.1% | 102.8 / 16.8 | 45 | S147S (TCG→TCC) | hyfD | hydrogenase 4 membrane subunit |
* | REL606 | 2,357,611 | 0 | A | C | 31.0% | 115.5 / 14.6 | 58 | T184P (ACC→CCC) | ackA | acetate kinase |
* | REL606 | 4,141,015 | 0 | A | C | 30.9% | 125.6 / 14.1 | 55 | T30P (ACC→CCC) | yijC | DNA‑binding transcriptional repressor |
* | REL606 | 157,994 | 0 | G | T | 30.6% | 174.9 / 34.8 | 72 | Q93K (CAA→AAA) | htrE | predicted outer membrane usher protein |
* | REL606 | 1,495,872 | 0 | G | A | 30.4% | 81.6 / 20.3 | 46 | S74L (TCG→TTG) | ansP | L‑asparagine transporter |
* | REL606 | 2,197,867 | 0 | T | G | 29.5% | 89.0 / 23.8 | 44 | A47A (GCA→GCC) | cirA | ferric iron‑catecholate outer membrane transporter |
* | REL606 | 92,359 | 0 | G | T | 29.4% | 83.0 / 14.7 | 34 | intergenic (+523/‑79) | fruR/yabB | DNA‑binding transcriptional dual regulator/hypothetical protein |
* | REL606 | 3,921,703 | 0 | T | G | 29.0% | 149.7 / 24.5 | 69 | intergenic (‑88/+111) | ppiC/ECB_03654 | peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/hypothetical protein |
* | REL606 | 60,574 | 0 | T | G | 28.8% | 127.4 / 10.0 | 52 | Y244S (TAC→TCC) | imp | organic solvent tolerance protein precursor |
* | REL606 | 1,777,009 | 0 | C | G | 28.8% | 135.3 / 21.3 | 52 | R3P (CGC→CCC) | rplT | 50S ribosomal protein L20 |
* | REL606 | 3,983,700 | 0 | T | G | 28.6% | 142.4 / 17.5 | 63 | T397P (ACC→CCC) | ECB_03713 | conserved hypothetical protein |
* | REL606 | 2,348,325 | 0 | A | C | 27.8% | 118.4 / 21.0 | 54 | G113G (GGT→GGG) | nuoA | NADH dehydrogenase subunit A |
* | REL606 | 1,019,082 | 0 | T | G | 27.4% | 138.5 / 21.4 | 62 | V62G (GTA→GGA) | ycbT | predicted fimbrial‑like adhesin protein |
* | REL606 | 1,564,941 | 0 | C | G | 27.3% | 117.3 / 10.9 | 44 | R29P (CGC→CCC) | ECB_01464 | putative fimbrial usher protein |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 499019 = | 80 (1.000) | 10 (0.140) | 6/92 | 8.6 | 13.8% | coding (3592/4281 nt) | rhsD | rhsD element protein |
? | REL606 | = 3099737 | 51 (0.700) | coding (409/2259 nt) | parC | DNA topoisomerase IV subunit A |