breseq  version 0.35.7  revision b7a44cd89118
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 34 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,584,9580TG61.5% ‑2.1 / 12.7 13intergenic (+14/‑104)suhB/yfhRinositol monophosphatase/predicted peptidase
*REL6064,549,4180TG34.7% 146.5 / 14.3 72T6P (ACC→CCC) yjiOmultidrug efflux system protein
*REL6064,335,1610TG34.2% 41.3 / 11.3 38intergenic (‑30/+29)yjdL/cadApredicted transporter/lysine decarboxylase 1
*REL6061,292,2590TC32.8% 114.0 / 23.7 67intergenic (+100/+44)galU/hnsglucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS
*REL6061,819,9590AC32.7% 113.8 / 10.0 52T121P (ACC→CCC) gdhAglutamate dehydrogenase
*REL6062800GC32.0% 98.7 / 11.7 50intergenic (+25/‑56)thrL/thrAthr operon leader peptide/bifunctional aspartokinase I/homeserine dehydrogenase I
*REL6062,526,1640GC31.1% 102.8 / 16.8 45S147S (TCG→TCChyfDhydrogenase 4 membrane subunit
*REL6062,357,6110AC31.0% 115.5 / 14.6 58T184P (ACC→CCC) ackAacetate kinase
*REL6064,141,0150AC30.9% 125.6 / 14.1 55T30P (ACC→CCC) yijCDNA‑binding transcriptional repressor
*REL606157,9940GT30.6% 174.9 / 34.8 72Q93K (CAA→AAA) htrEpredicted outer membrane usher protein
*REL6061,495,8720GA30.4% 81.6 / 20.3 46S74L (TCG→TTG) ansPL‑asparagine transporter
*REL6062,197,8670TG29.5% 89.0 / 23.8 44A47A (GCA→GCCcirAferric iron‑catecholate outer membrane transporter
*REL60692,3590GT29.4% 83.0 / 14.7 34intergenic (+523/‑79)fruR/yabBDNA‑binding transcriptional dual regulator/hypothetical protein
*REL6063,921,7030TG29.0% 149.7 / 24.5 69intergenic (‑88/+111)ppiC/ECB_03654peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/hypothetical protein
*REL60660,5740TG28.8% 127.4 / 10.0 52Y244S (TAC→TCC) imporganic solvent tolerance protein precursor
*REL6061,777,0090CG28.8% 135.3 / 21.3 52R3P (CGC→CCC) rplT50S ribosomal protein L20
*REL6063,983,7000TG28.6% 142.4 / 17.5 63T397P (ACC→CCC) ECB_03713conserved hypothetical protein
*REL6062,348,3250AC27.8% 118.4 / 21.0 54G113G (GGT→GGGnuoANADH dehydrogenase subunit A
*REL6061,019,0820TG27.4% 138.5 / 21.4 62V62G (GTA→GGA) ycbTpredicted fimbrial‑like adhesin protein
*REL6061,564,9410CG27.3% 117.3 / 10.9 44R29P (CGC→CCC) ECB_01464putative fimbrial usher protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 499019 =80 (1.000)10 (0.140) 6/92 8.6 13.8% coding (3592/4281 nt) rhsD rhsD element protein
?REL606 = 3099737 51 (0.700)coding (409/2259 nt) parC DNA topoisomerase IV subunit A