Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,584,9580TG61.5% ‑2.1 / 12.7 13intergenic (+14/‑104)suhB/yfhRinositol monophosphatase/predicted peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (8/0);  total (8/5)
Fisher's exact test for biased strand distribution p-value = 7.77e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCAATCACCCGCCCCCGCC  >  REL606/2584914‑2584981
                                            |                       
tGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATatc                   <  1:5207529/51‑1 (MQ=255)
  aCGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATatcaa                 <  1:5096741/51‑1 (MQ=255)
  aCGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGGGATatcaa                 >  1:5005848/1‑51 (MQ=255)
     aGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGGGATatcaa                 >  1:2953277/1‑48 (MQ=255)
      gTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCAATCAc             <  1:1361448/51‑1 (MQ=255)
        tAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCAATCAccc           <  1:3118255/51‑1 (MQ=255)
        tAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGGGATATCAATCAccc           >  1:5815807/1‑51 (MQ=255)
               cGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCAATCAcccgcccccg    <  1:6920380/51‑1 (MQ=255)
               cGCTCTGAAGCGTTAATGACTCAGGCGGGGGATATCAATCAcccgcccccc    >  1:4465651/1‑50 (MQ=255)
                 ctctGAAGCGTTAATGACTCAGGCGGGGGGTATTAATCAaccccccccccc  >  1:653444/1‑51 (MQ=19)
                 ctctGAAGCGTTAATGACTCAGGCGGGGGGTATCAATCAcccgccccc     >  1:2909260/1‑48 (MQ=38)
                 ctctGAAGCGTTAATGACTCAGGCGGGGGATATCAATCAcccgccccc     >  1:2930244/1‑48 (MQ=255)
                   ctGAAGCGTTAATGACTCAGGCGGGGGATATCAATCAcccgcccc      >  1:5106776/1‑45 (MQ=255)
                                            |                       
TGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCAATCACCCGCCCCCGCC  >  REL606/2584914‑2584981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑