| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,335,161 | 0 | T | G | 34.2% | 41.3 / 11.3 | 38 | intergenic (‑30/+29) | yjdL/cadA | predicted transporter/lysine decarboxylase 1 |
| Reads supporting (aligned to +/- strand): ref base T (5/20); new base G (0/13); total (5/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAA‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTC > REL606/4335111‑4335211 | cgGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAA‑Gt < 1:384140/51‑1 (MQ=255) gCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAA‑GTgg < 1:2131633/51‑1 (MQ=255) tgtgAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAA‑GTGGCa < 1:1678768/51‑1 (MQ=255) tgtgAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAA‑GTGGCa < 1:2395914/51‑1 (MQ=255) tgAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAA‑GTGGCAAg < 1:5879666/51‑1 (MQ=255) aGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAA‑GTGGCaa > 1:6454308/1‑48 (MQ=255) aGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAA‑GTGGCAAGcc < 1:2093806/51‑1 (MQ=255) aTGTGTTCTCCTTATGAGCAAAAAAGGGAA‑GTGGCAAGCCACTTCCCTTGt < 1:5365406/51‑1 (MQ=255) tgtaTTCTCCTTATGAGCAAAAAAGGGAA‑GTGGCAAGCCACTTCCCTTGTa < 1:3304997/51‑1 (MQ=255) tctcCTTATGAGCAAAAAAGGGAA‑GTGGCAAGCCACTTCCCTTGTACGAGc < 1:5115531/51‑1 (MQ=255) ttATGAGAAAAAAAGGGAA‑GGGGAAAGCCACTTCCCTTGTACGAGCTAatt < 1:5524840/51‑1 (MQ=255) gAGCAAAAAAGGGAA‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTAttt < 1:6906414/51‑1 (MQ=255) gAGCAAAAAAGGGAA‑GGGGAAAGCCACTTCCCTTGTACGAGCTna < 1:6601976/45‑1 (MQ=255) aaaaagGGGAG‑GGGGCAAGCCACTTCCCTTGTACGAGCt < 1:1548118/39‑1 (MQ=255) aaaaagGGGAA‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTAttttt < 1:6137118/49‑1 (MQ=255) aaaaagGGAAA‑GGGGAAAGCCACTTCCCTTGTACGAGCTaa < 1:14813/41‑1 (MQ=255) aaaaaaGGGAG‑GGGGAAAGCCACTTCCCTTGTACGAGCTAATTAtttttt < 1:266969/50‑1 (MQ=255) aaaaaaGGGAAGGGGGAAAGCCACTTCCCTTGTACGAGCTAATTAttt < 1:664767/48‑1 (MQ=255) aaaaaaGGGAA‑GTGGCAAGCCACTTCCCTTGTACGAGCTAattatt < 1:3039265/46‑1 (MQ=255) aaaaaaGGGAA‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTAtttttt < 1:4240626/50‑1 (MQ=255) aaaaaaGGGAA‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTAtttttt < 1:4600036/50‑1 (MQ=255) aaaaaaGGGAA‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTAttttt < 1:4683655/49‑1 (MQ=255) aaaaaaGGGAA‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTg < 1:3891388/51‑1 (MQ=255) aaaaaaGGGAA‑GTGGAAAGCCACTTCCCTTGTACGAGCTAATTAttttt < 1:4224562/49‑1 (MQ=255) aaaaaaGGGAA‑GTGGAAAGCCACTTCCCTTGTACGAGCTAATTAtttt < 1:5568120/48‑1 (MQ=255) aaaaaaGGGAA‑GTGGAAAGCCACTTCCCTTGTACGAGCTAATTAttt < 1:2918992/47‑1 (MQ=255) aaaaaaGGGAA‑GGGGCAAGCCACTTCCCTTGTACGAGCTAattat < 1:4415142/45‑1 (MQ=255) aaaaaaGGGAA‑GGGGAAAGCCACTTCCCTTGTACGAGc < 1:2119312/38‑1 (MQ=38) aaaaaaGGGAA‑GGGGAAAGCCACTTCCCTTGTACGAGCTAatta < 1:1771533/44‑1 (MQ=38) aaaaaaGGGAA‑GGGGAAAGCCACTTCCCTTGTACGAGCTAATTAtttttt < 1:736136/50‑1 (MQ=255) aaaaaaGGGAA‑GGGGAAAGCCACTTCCCTTGTACGAGCTAATTAttttt < 1:2220817/49‑1 (MQ=255) aaaaaGGGAAGGGGGAAAGCCACTTCCCTTGTACGAGCTaa < 1:279505/41‑1 (MQ=255) aaGGGAA‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTAtttttt > 1:4110775/1‑46 (MQ=255) gggAA‑GGGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTttct < 1:2431038/51‑1 (MQ=255) aa‑GTGGCAAGCCACTTGCCTTGTACGAGCTAATTATTTTTTGCTTtcttct < 1:6083750/51‑1 (MQ=255) aa‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTtcttct > 1:3684460/1‑51 (MQ=255) aa‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTtcttct > 1:7152164/1‑51 (MQ=255) a‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTtcttctc < 1:4202587/51‑2 (MQ=255) a‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTcttctt > 1:3117760/1‑51 (MQ=255) tGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTc > 1:2146139/1‑51 (MQ=255) | CGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGAGCAAAAAAGGGAA‑GTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTC > REL606/4335111‑4335211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |