| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 92,359 | 0 | G | T | 29.4% | 83.0 / 14.7 | 34 | intergenic (+523/‑79) | fruR/yabB | DNA‑binding transcriptional dual regulator/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (6/18); new base T (10/0); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.11e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGG > REL606/92309‑92409 | ttAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTg < 1:3223324/51‑1 (MQ=255)ttAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTg < 1:7013312/51‑1 (MQ=255) aaaCATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCtt < 1:168523/51‑1 (MQ=255) aCATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCtttt < 1:7087674/51‑1 (MQ=255) cATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTtc < 1:6084983/51‑1 (MQ=255) cATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTtc < 1:3211015/51‑1 (MQ=255) cGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTtcctc < 1:38355/51‑1 (MQ=255) ttttttACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCctccgct < 1:3778374/51‑1 (MQ=255) ttttttACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCctccgct < 1:51173/51‑1 (MQ=255) tttttACCTCGGTATGCCTTGTGACTGGCTTTTCAAACTTTttctcc > 1:5382843/1‑47 (MQ=255) ttttACCTCGGTATGCCTTGTGACTGGCTTTTCAAGCtttt > 1:1437174/1‑41 (MQ=38) ttttACCTCGGTATGCCTTGTGACTGGCTTTACAAGCTTTTTctcccctcc > 1:4491599/1‑51 (MQ=255) ttttACCTCGGTATGCCTTGTGACTGGCTTTACAAACTTTTTctcccctcc > 1:5058527/1‑51 (MQ=255) ttACCTCGGTATGCCTTGTGACTGGCTTTTCAAACTTTttctcc > 1:3833704/1‑44 (MQ=25) ttACCTCGGTATGCCTTGTGACTGGCTTTAAAAACTTTttctcc > 1:352188/1‑44 (MQ=25) ttACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGt < 1:2593707/51‑1 (MQ=255) tACCTCGGTATGCCTTGTGACTGGCTTTACAAGCTTTttctcc > 1:1525224/1‑43 (MQ=255) tACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTa < 1:713301/51‑1 (MQ=255) aCCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTaa < 1:5803693/51‑1 (MQ=255) cTCGGTATGCCTTGTGACTGGCTTTTCAAACTTTtcctcc > 1:1480503/1‑40 (MQ=37) cTCGGTATGCCTTGTGACTGGCTTTACAAGGTTTTTctcccctcc > 1:2374851/1‑45 (MQ=255) ggTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTcc < 1:3729070/51‑1 (MQ=255) gTATGCCTTGTGACTGGCTTTTCAAGCtttt > 1:2388268/1‑31 (MQ=25) aTGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCttt < 1:3636916/51‑1 (MQ=255) ttGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTg < 1:6022422/51‑1 (MQ=255) tgACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGa < 1:3546907/51‑1 (MQ=255) gACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGaa < 1:6271157/51‑1 (MQ=255) ggCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATtg < 1:7034162/51‑1 (MQ=255) gCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATtgt > 1:363315/1‑51 (MQ=255) cTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATtgtg > 1:5101418/1‑51 (MQ=255) cTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATtgtg > 1:2368207/1‑51 (MQ=255) ttGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTgg < 1:1074680/51‑1 (MQ=255) ttGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTgg < 1:4513345/51‑1 (MQ=255) ttGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTgg > 1:2942215/1‑51 (MQ=255) tGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTggg > 1:6996787/1‑51 (MQ=255) tGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTggg > 1:5359101/1‑51 (MQ=255) gACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTgggg < 1:2753590/51‑1 (MQ=255) gACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTgggg > 1:4230497/1‑51 (MQ=255) | TTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGG > REL606/92309‑92409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |