YPD Medium

General media used for culturing Yeast. Adapted from Cold spring harbor

In 1 L bottle:

1L Component
20g Peptone
10 g Yeast Extract

  • Dissolve above in ~900 mL water.
  • Bring to 950 mL (final volume will be 1L after autoclaving)

In separate 500mL bottle, prepare 40% Glucose w/v

Autoclave separately. WARNING If glucose added before autoclaving, media will carmelize and be unusable.

  • Add 50 mL of 40% glucose solution to YPD media.

-- Daniel Deatherage - 2021-04-19

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Topic revision: r1 - 2021-04-19 - 21:13:07 - Main.DanielDeatherage
 
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