YPD Medium

General media used for culturing Yeast. Adapted from Cold spring harbor

In 1 L bottle:

1L Component
20g Peptone
10 g Yeast Extract

  • Dissolve above in ~900 mL water.
  • Bring to 950 mL (final volume will be 1L after autoclaving)

In separate 500mL bottle, prepare 40% Glucose w/v

Autoclave separately. WARNING If glucose added before autoclaving, media will caramelize and be unusable.

  • Add 50 mL of 40% glucose solution to YPD media.
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Contributors to this topic Edit topic JeffreyBarrick, DanielDeatherage
Topic revision: r3 - 2022-06-01 - 18:44:45 - Main.JeffreyBarrick
 
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