YPD Medium
General media used for culturing Yeast. Adapted from
Cold spring harbor
In 1 L bottle:
| 1L |
Component |
| 20g |
Peptone |
| 10 g |
Yeast Extract |
- Dissolve above in ~900 mL water.
- Bring to 950 mL (final volume will be 1L after autoclaving)
In separate 500mL bottle, prepare 40% Glucose w/v
Autoclave separately.
WARNING If glucose added before autoclaving, media will caramelize and be unusable.
- Add 50 mL of 40% glucose solution to YPD media.