R2A

R2A is a medium that can be used to grow a wide variety of soil microbes.

1L 5L Component
0.5 g 2.5 g Yeast Extract
0.5 g 2.5 g Proteose Peptone No. 3
0.5 g 2.5 g Casamino Acids
0.5 g 2.5 g Dextrose
0.5 g 2.5 g Soluble Starch
0.3 g 1.5 g Sodium Pyruvate
0.3 g 1.5 g Dipotassium Phosphate
0.05 g 0.25 g Magnesium Sulfate
Add dH2O to final volume and autoclave.

If making R2A solid media also add 15 g of agar and 1 ml of 5% antifoam per liter.

Edit | Attach | Watch | Print version | History: r2 < r1 | Backlinks | Raw View | More topic actions

 Barrick Lab  >  ProtocolsMediaRecipes  >  ProtocolsRecipesR2A

Contributors to this topic Edit topic CraigBarnhart, JeffreyBarrick
Topic revision: r2 - 2015-06-09 - 16:06:11 - Main.JeffreyBarrick
Lab.ProtocolsRecipesR2A moved from Lab.ProtocolsRecipesR2a on 2015-06-09 - 16:04 by Main.JeffreyBarrick -
 
This site is powered by the TWiki collaboration platform Powered by Perl This site is powered by the TWiki collaboration platformCopyright ©2024 Barrick Lab contributing authors. Ideas, requests, problems? Send feedback