R2A

R2A is a medium that can be used to grow a wide variety of soil microbes.

1L 5L Component
0.5 g 2.5 g Yeast Extract
0.5 g 2.5 g Proteose Peptone No. 3
0.5 g 2.5 g Casamino Acids
0.5 g 2.5 g Dextrose
0.5 g 2.5 g Soluble Starch
0.3 g 1.5 g Sodium Pyruvate
0.3 g 1.5 g Dipotassium Phosphate
0.05 g 0.25 g Magnesium Sulfate
Add dH2O to final volume and autoclave.

If making R2A solid media also add 15 g of agar and 1 ml of 5% antifoam per liter.

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 Barrick Lab  >  ProtocolsMediaRecipes  >  ProtocolsRecipesR2A

Contributors to this topic Edit topic JeffreyBarrick, CraigBarnhart
Topic revision: r2 - 2015-06-09 - 16:06:11 - Main.JeffreyBarrick
Lab.ProtocolsRecipesR2A moved from Lab.ProtocolsRecipesR2a on 2015-06-09 - 16:04 by Main.JeffreyBarrick -
 
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