Difference: ProtocolsRecipesR2A (1 vs. 2)

Revision 22015-06-09 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"
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R2A

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R2A is a medium that can be used to grow a wide variety of soil microbes.
 
1L 5L Component
0.5 g 2.5 g Yeast Extract
0.5 g 2.5 g Proteose Peptone No. 3
0.5 g 2.5 g Casamino Acids
0.5 g 2.5 g Dextrose
0.5 g 2.5 g Soluble Starch
0.3 g 1.5 g Sodium Pyruvate
0.3 g 1.5 g Dipotassium Phosphate
0.05 g 0.25 g Magnesium Sulfate
Add dH2O to final volume and autoclave.
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If making R2A solid media also add 15 g of agar and 1 ml of 5% antifoam per liter:
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If making R2A solid media also add 15 g of agar and 1 ml of 5% antifoam per liter.
 
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META TOPICMOVED by="JeffreyBarrick" date="1433865893" from="Lab.ProtocolsRecipesR2a" to="Lab.ProtocolsRecipesR2A"
 

Revision 12011-07-29 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

R2A

1L 5L Component
0.5 g 2.5 g Yeast Extract
0.5 g 2.5 g Proteose Peptone No. 3
0.5 g 2.5 g Casamino Acids
0.5 g 2.5 g Dextrose
0.5 g 2.5 g Soluble Starch
0.3 g 1.5 g Sodium Pyruvate
0.3 g 1.5 g Dipotassium Phosphate
0.05 g 0.25 g Magnesium Sulfate
Add dH2O to final volume and autoclave.

If making R2A solid media also add 15 g of agar and 1 ml of 5% antifoam per liter:

 
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