Barrick Lab :: ProtocolsRecipesMinimalGlucose

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---+++ MG:  Minimal Glucose agar, aka DM: Davis Mingioli agar
*%RED%Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately. Compounds that inhibit growth are produced when [[http://schaechter.asmblog.org/schaechter/2014/12/the-great-plate-count-anomaly-that-is-no-more.html][agar and phosphate]] or phosphate and glucose are autoclaved together.%ENDCOLOR%*

|  *1L*  |  *1.5L*  |  *Component*  |  *MW*  |
|  5.3 g  |  8 g  | Potassium Phosphate (dibasic) K<sub>2</sub>HPO<sub>4</sub> | MW 174.18 |  
|  2 g  | 3 g | Potassium Phosphate (monobasic) KH<sub>2</sub>PO<sub>4</sub> | MW 136.07 |
|  1 g  | 1.5 g | Ammonium Sulfate (NH<sub>4</sub>)<sub>2</sub> SO<sub>4</sub>| MW 132.08 |
|  0.5 g  | 0.75 g | Sodium Citrate (trisodium, dihydrate) Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>  (H<sub>2</sub>O)<sub>2</sub>| MW 294.10 |
|  333 ml    | 500 ml | dH<sub>2</sub>O | |
Autoclave. <br>

Next, prepare the agar: <br>

|  *1L*  |  *1.5L*  |  *Component*  | 
| 16 g | 24 g | Agar |
| 1 ml | 1.5 ml | Antifoam (5%) |
| 333 ml | 500 ml | dH<sub>2</sub>O |
Autoclave. <br>

Next, prepare the sugar. The final concentration is 0.4% w/v: <br>

|  *1L*  |  *1.5L*  |  *Component*  | 
| 4 g | 6 g | Glucose (or other sugar) |
|  333 ml | 500 ml | dH<sub>2</sub>O |
Autoclave. <br>

After the 3 parts have been autoclaved, combine the contents of the three flasks together while they are still warm add the following stock solutions: <br>

|  *1L*  |  *1.5L*  |  *Component*  |
| 1.0 ml | 1.5 ml | 10% Magnesium Sulfate MgSO<sub>4</sub> (separately autoclaved stock)  |
| 1.0 ml | 1.5 ml | 0.2% Thiamin (vitamin B1) (filter sterilized stock) |

Source: [[http://myxo.css.msu.edu/ecoli/dmagar.html][Lenski Lab Protocol]]

Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesMinimalGlucose

Contributors to this topic

JeffreyBarrick, CraigBarnhart, AurkoDasgupta

Topic revision: r10 - 2020-09-26 - 23:46:47 - Main.JeffreyBarrick